A tensile trilayered cytoskeletal endotube drives capillary-like lumenogenesis
| Autor: | Hongjie Zhang, Gholamali Jafari, Liakot A. Khan, Verena Gobel, Edward Membreno, Siyang Yan, Nan Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Endosome
Morphogenesis Intermediate Filaments Lumen (anatomy) Biology Article 03 medical and health sciences 0302 clinical medicine Intermediate Filament Proteins Tubulin Animals Cytoskeleton Intermediate filament Caenorhabditis elegans Caenorhabditis elegans Proteins Actin Research Articles 030304 developmental biology 0303 health sciences Vesicle Cell Biology Actins Capillaries Cytoskeletal Proteins biology.protein Biophysics RNA Interference 030217 neurology & neurosurgery |
| Zdroj: | The Journal of Cell Biology |
| ISSN: | 1540-8140 0021-9525 |
| Popis: | Unicellular tubes, components of organs and capillaries, form intracellular lumens. Khan et al. report that a tensile triple intermediate-filament lattice, embedded between microfilaments and microtubules, tunnels the lumen through the single-cell C. elegans excretory canal and transforms concentric lumen expansion into lumen forward extension. Unicellular tubes are components of internal organs and capillaries. It is unclear how they meet the architectural challenge to extend a centered intracellular lumen of uniform diameter. In an RNAi-based Caenorhabditis elegans screen, we identified three intermediate filaments (IFs)—IFA-4, IFB-1, and IFC-2—as interactors of the lumenal membrane-actin linker ERM-1 in excretory-canal tubulogenesis. We find that IFs, generally thought to affect morphogenesis indirectly by maintaining tissue integrity, directly promote lumenogenesis in this capillary-like single-cell tube. We show that ERM-1, ACT-5/actin, and TBB-2/tubulin recruit membrane-forming endosomal and flux-promoting canalicular vesicles to the lumen, whereas IFs, themselves recruited to the lumen by ERM-1 and TBB-2, restrain lateral vesicle access. IFs thereby prevent cystogenesis, equilibrate the lumen diameter, and promote lumen forward extension. Genetic and imaging analyses suggest that IFB-1/IFA-4 and IFB-1/IFC-2 polymers form a perilumenal triple IF lattice, sandwiched between actin and helical tubulin. Our findings characterize a novel mechanism of capillary-like lumenogenesis, where a tensile trilayered cytoskeletal endotube transforms concentric into directional growth. |
| Databáze: | OpenAIRE |
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