Increased apoptosis, changes in intracellular Ca2+, and functional alterations in lymphocytes and macrophages after in vitro exposure to static magnetic field
| Autor: | Pinsky C, LaBella Fs, Le Boulaire C, Denis Flipo, Krzysztof Krzystyniak, Philippe P. Roux, Benquet C, Michel Fournier |
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| Rok vydání: | 1998 |
| Předmět: |
Interleukin 2
Intracellular Fluid Health Toxicology and Mutagenesis Lymphocyte Spleen Apoptosis In Vitro Techniques Toxicology Mice Electromagnetic Fields Phagocytosis medicine Macrophage Animals Lymphocytes biology Macrophages Molecular biology Thymocyte medicine.anatomical_structure Concanavalin A Immunology biology.protein Interleukin-2 Calcium Intracellular medicine.drug |
| Zdroj: | ResearcherID |
| ISSN: | 1528-7394 |
| Popis: | Electromagnetic-related alteration of cellular functions is well documented for extremely low-frequency low-energy pulsing electromagnetic fields (ELF-EMF). In this study we examined the in vitro effects of static magnetic fields (SMF) on the cellular immune parameters of the C57BI/6 murine macrophages, spleen lymphocytes, and thymic cells. The cells were exposed in vitro for 24 h at 37 degrees C, 5% CO2, to 250-1500 G SMF. Exposure to the SMF resulted in the decreased phagocytic uptake of fluorescent latex microspheres, which was accompanied by an increased intracellular Ca2+ level in macrophages. Exposure to SMF decreased mitogenic responses in lymphocytes, as determined by incorporation of [3H]thymidine into the cells. This was associated with the increased Ca2+ influx in concanavalin A-stimulated lymphocytes. Furthermore, exposure to SMF produced markedly increased apoptosis of thymic cells, as determined by flow cytometry. Overall, in vitro exposure of immunocompetent cells to 250-1500 G SMF altered several functional parameters of C57BI/6 murine macrophages, thymocytes, and spleen lymphocytes. |
| Databáze: | OpenAIRE |
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