Cloning and Sequencing of the Sphingomonas (Pseudomonas) paucimobilis Gene Essential for the O Demethylation of Vanillate and Syringate
| Autor: | Shinya Kawai, Yoshihiro Katayama, Takahiro Obi, Seiji Nishikawa, Shoko Kubota, Tatsuhide Kasahara, Tomonori Sonoki, Noriyuki Morohoshi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: |
DNA
Bacterial Sphingomonas paucimobilis Mutant Molecular Sequence Data Genetics and Molecular Biology Molecular cloning Applied Microbiology and Biotechnology Lignin Gallic Acid Pseudomonas Amino Acid Sequence Cloning Molecular Demethylation Vanillic Acid Ecology biology Nucleic acid sequence biology.organism_classification Sphingomonas Open reading frame Biochemistry Dealkylation Genes Bacterial Food Science Biotechnology |
| Zdroj: | Scopus-Elsevier |
| Popis: | Sphingomonas ( Pseudomonas ) paucimobilis SYK-6 is able to grow on 5,5′-dehydrodivanillic acid (DDVA), syringate, vanillate, and other dimeric model compounds of lignin as a sole carbon source. Nitrosoguanidine mutagenesis of S. paucimobilis SYK-6 was performed, and two mutants with altered DDVA degradation pathways were isolated. The mutant strain NT-1 could not degrade DDVA, but could degrade syringate, vanillate, and 2,2′,3′-trihydroxy-3-methoxy-5,5′-dicarboxybiphenyl (OH-DDVA). Strain DC-49 could slowly assimilate DDVA, but could degrade neither vanillate nor syringate, although it could degrade protocatechuate and 3- O -methylgallate. A complementing DNA fragment of strain DC-49 was isolated from the cosmid library of strain SYK-6. The minimum DNA fragment complementing DC-49 was determined to be the 1.8-kbp insert of pKEX2.0. Sequencing analysis showed an open reading frame of 1,671 bp in this fragment, and a similarity search indicated that the deduced amino acid sequence of this open reading frame had significant similarity (60%) to the formyltetrahydrofolate synthetase of Clostridium thermoaceticum. |
| Databáze: | OpenAIRE |
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