Directional bioconversion and optimization of stevioside into rubusoside by Lelliottia sp. LST-1
| Autor: | Jing Lin, Ziyang Ma, Xiufang Hu, Junhao Sang, Ou Li, Jiaqi Xin, Ke Huang, Guohong Zeng, zhiyun Hong, Jingyu Huang, Hongwei Li |
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| Rok vydání: | 2022 |
| Předmět: |
food.ingredient
Bioconversion Steviol Applied Microbiology and Biotechnology Hydrolysis chemistry.chemical_compound food Glucosides Biotransformation Food science Stevioside Aftertaste Phylogeny chemistry.chemical_classification biology Chemistry Food additive Glycoside General Medicine Enzyme assay Stevia rebaudiana Transformation (genetics) Enzyme biology.protein Diterpenes Kaurane Biotechnology |
| Zdroj: | Journal of Applied Microbiology. 132:1887-1899 |
| ISSN: | 1365-2672 1364-5072 |
| Popis: | Aims The present study aimed to specifically transform stevioside (ST) into rubusoside (RS) through bioconversion with high efficiency, seeking to endow steviol glycosides (SGs) with subtle flavours for commercial acceptability. Methods and Results An endophytic bacterium named Lelliottia LST-1 was screened and confirmed to specifically convert ST into RS, reaching a conversion rate of 75.4% after response surface optimization. Phylogenetic analysis combined with complete genome sequencing demonstrated that LST-1 was also presumed to be a new species. To further explore the principle and process of biological transformation, the potential beta-glucosidases GH3-1, GH3-2, GH3-3 and GH3-4 were expressed, purified and reacted with SGs. High-performance liquid chromatography revealed that all enzymes hydrolysed ST and generated RS, but substrate specificity analysis indicated that GH3-2 had the highest substrate specificity towards STs and the highest enzyme activity. Conclusion The potential β-glucosidase GH3-2 in Lelliottia sp. LST-1 was found to specifically and efficiently convert ST to RS. Significance and Impact of Study The efficient biotransformation of ST into RS will be beneficial to its large-scale production and extensive application in the food and pharmaceutical industries. |
| Databáze: | OpenAIRE |
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