Intestinal changes and performance parameters in ticks feeding on calves immunized with subunits of immunogens against Rhipicephalus microplus
| Autor: | Joaquín Hernán Patarroyo Salcedo, Leandro Dias Araújo, Cintia F. Fidelis, Marlene I. Vargas, Ricardo Wagner Portela, Jesús A. Cortés-Vecino, Gabriel Andres Tafur-Gómez, Pablo A. Prates-Patarroyo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Immunogen Histology Ixodidae Histología animal Cattle Diseases Ganado Tick Tick control 01 natural sciences 030308 mycology & parasitology Microbiology 03 medical and health sciences Midgut parasitic diseases Bovina Rhipicephalus Animals Metastigmata Tick Control Antigens Cattle tick 0303 health sciences Vaccines Ecology biology General Medicine biology.organism_classification Bos taurus Tick Infestations Intestines 010602 entomology Animal ecology Insect Science Proteínas Rhipicephalus microplus Cattle Immunization Tick proteins |
| Zdroj: | Repositorio Institucional UDCA Universidad de Ciencias Aplicadas y Ambientales U.D.C.A instacron:Universidad de Ciencias Aplicadas y Ambientales U.D.C.A |
| Popis: | We describe the intestinal changes and biological parameters of the tick species Rhipicephalus microplus exposed to the immune response of calves vaccinated with two subunits of immunogens. The first group of Bos taurus calves was immunized with a synthetic peptide (SBm7462), whereas the second group received an inoculum for synthetic control. The third group was immunized with a recombinant peptide (rSBm7462); an inoculum was injected into a fourth group of calves for recombinant control. Each formulation was administered to these calves during three times at intervals of 30 days. At 21 days after the last immunization, the calves were challenged using a total of 4500 larvae per animal. Indirect ELISA was realized to identify the kinetics of IgGs from samples of calves studied. Naturally detaching ticks were collected for analyses of biological performance and histological changes in the midgut. We dissected randomly detached ticks. The midgut of each of these ticks was removed and processed routinely for histology, stained with hematoxylin-eosin (H&E) and slow Giemsa. Slides were also subjected to immunohistochemistry. The antibody response showed significant induction of high-affinity IgGs in calves immunized with both peptides in comparison to calves of the control groups. Histological changes included damage of the intestinal epithelium in ticks fed on immunized hosts and intense immunostaining in midgut cells, using the serum of calves immunized with recombinant peptide. There were significant differences in all biological performing parameters of ticks detached from vaccinated calves in comparison with ticks of the control groups. We identified reductions of 87.7 and 93.5% in engorged ticks detached from calves immunized with a synthetic and recombinant peptides, respectively, a 28 and 8.60% lower egg mass in groups immunized with synthetic and recombinant peptides, respectively, and a 38.4% reduction of the value of nutrient index/tick in the group immunized with the recombinant peptide. Our findings show that the immune response induced by small peptides in cattle can modify the digestion and metabolism of ticks fed on vaccinated animals, resulting in changes in tick performance. |
| Databáze: | OpenAIRE |
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