TRIM44 facilitates ovarian cancer proliferation, migration, and invasion by inhibiting FRK
| Autor: | Mingrong Qie, Ke Yi, Xiu-Zhang Yu, Jialing Yuan, Hui Ye, Minmin Hou |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Cancer Research
Mice Nude Theoretical research Biology Tripartite Motif Proteins Mice Western blot In vivo Cell Movement Cell Line Tumor medicine Animals Humans MTT assay Cell Proliferation Ovarian Neoplasms medicine.diagnostic_test Intracellular Signaling Peptides and Proteins Protein-Tyrosine Kinases medicine.disease Neoplasm Proteins Gene Expression Regulation Neoplastic Oncology Tumor progression Cell culture Cancer research Female Cancer development Ovarian cancer |
| Zdroj: | Neoplasma. 68(4) |
| ISSN: | 0028-2685 |
| Popis: | Ovarian cancer (OC) is the leading cause of gynecologic cancer-related death in the world. Accumulating evidence indicated the important role of TRIM44 in cancer development. However, how TRIM44 displays in OC and the underlying mechanism remained unclear. TRIM44 and FRK expression in OC tissues and cell lines were investigated by western blot and RT-qPCR. Histotype of tissue samples and patients' data were analyzed. Kaplan-Meier Curve was performed to validate the effect of TRIM44. Colony formation assay, MTT assay, Transwell assay, and wound-healing assay were applied to elucidate the function of TRIM44 in OC cells. CHIP assay was used to explore the association between TRIM44 and FRK. Finally, we performed SKOV3 xenografts in Balb/c nude mice to further confirm the involvement of TRIM44 in OC development. We found TRIM44 highly expressed while FRK displayed low expression in OC cell lines and tissues. Moreover, analysis of histotype of tissues and patients' data and Kaplan-Meier Curve implied the important role of TRIM44 and FRK in tumor progression. Further in vitro study suggested that knocking down TRIM44 inhibited OC cells proliferation, migration, and invasion. Besides, FRK was identified as the target gene of TRIM44 in OC, and TRIM44 promoted OC cells proliferation, migration, and invasion by inhibiting FRK. Finally, in vivo animal experiment further confirmed the promotive effect of TRIM44 on OC progression. Our findings demonstrated that TRIM44 facilitated OC proliferation, migration, and invasion by inhibiting FRK, providing new insights for theoretical research and therapy of OC. |
| Databáze: | OpenAIRE |
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