Prostaglandin-mediated effects in early canine corpus luteum: In vivo effects on vascular and immune factors

Autor: Renata Nowaczyk, Miguel Tavares Pereira, Bernd Hoffmann, Tomasz Janowski, Aykut Gram, Alois Boos, Mariusz P. Kowalewski
Přispěvatelé: University of Zurich, Kowalewski, Mariusz P
Rok vydání: 2018
Předmět:
0301 basic medicine
endocrine system
medicine.medical_specialty
10077 Institute of Veterinary Anatomy
media_common.quotation_subject
Prostaglandin
Biology
Luteal phase
Angiopoietin
1309 Developmental Biology
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
Dogs
4-Butyrolactone
In vivo
Corpus Luteum
Internal medicine
medicine
Dog (Canis lupus familiaris)
Animals
Immunologic Factors
RNA
Messenger
Sulfones
11434 Center for Clinical Studies
Receptor
Ovulation
media_common
030219 obstetrics & reproductive medicine
Cyclooxygenase 2 Inhibitors
Vascularization
1310 Endocrinology
030104 developmental biology
medicine.anatomical_structure
Immune system
chemistry
Gene Expression Regulation
Receptors
Estrogen
Prostaglandins
570 Life sciences
biology
Animal Science and Zoology
Female
1103 Animal Science and Zoology
Endothelin receptor
Transcriptome
Corpus luteum
Developmental Biology
Zdroj: Reproductive biology. 19(1)
ISSN: 2300-732X
Popis: Prostaglandins (PGs) are important regulators of the early corpus luteum (CL) in the dog. Whereas, initially, CL is gonadotropin independent, in the second half of its lifespan, hypophyseal support is required. The transition period is marked by decreased availability of PGs, in particular of PGE2. We previously reported that inhibition of COX2/PTGS2 in vivo suppressed luteal production of PGE2, lowered circulating progesterone and negatively affected luteal development. Therefore, bitches were treated with a COX2-specific blocker, firocoxib, for 5, 10, 20 and 30 days after ovulation, leading to suppression of the steroidogenic machinery. Control groups received a placebo for the same periods. Considering the wide range of possible modulatory roles of PGs shown in different organ systems, this follow-up project aimed to understand further possible PG-mediated effects in early canine CL. Thirty-four (34) factors related predominantly to vascularization and immune response were screened (mRNAs and proteins) on samples from the above described in vivo study. Most of the effects were observed during the transitional period (days 20 and 30). The inhibition of COX2 diminished the expression of angiopoietin family members ANGPT1, -2, Tie1 and -2 receptors. The expression of endothelin (ET)-1 was increased. Concerning the immune system, increased expression of the pro-inflammatory cytokines, IL1β, IL6 and IL12a, and elevated expression levels of CD4, was observed. Cumulatively, besides its involvement in regulating steroidogenesis, our results indicate a broader role of PGs in the canine CL, including modulation of angiogenesis, vascular stabilization and local immunomodulation. Possible cross-species translational effects are strongly implied.
Databáze: OpenAIRE
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