Molecular genetic variability of commercial and wild accessions of passion fruit (Passiflora spp.) targeting ex situ conservation and breeding
| Autor: | C. B. M. Cerqueira-Silva, Gustavo Maruyama Mori, João G. P. Vieira, Ronan Xavier Corrêa, Onildo Nunes de Jesus, Elisa Susilene Lisboa dos Santos, Anete Pereira de Souza |
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| Rok vydání: | 2014 |
| Předmět: |
Germplasm
single sequence repeats Passifloraceae molecular markers Breeding Catalysis Article lcsh:Chemistry Inorganic Chemistry Passiflora Species Specificity Botany Cluster Analysis Genetic variability Physical and Theoretical Chemistry lcsh:QH301-705.5 Molecular Biology Spectroscopy Alleles Passiflora cincinnata Genetic diversity Polymorphism Genetic biology Organic Chemistry Gene Amplification Genetic Variation Bayes Theorem General Medicine genetic diversity germplasm Ex situ conservation biology.organism_classification Computer Science Applications lcsh:Biology (General) lcsh:QD1-999 Fruit Genetic structure Microsatellite Microsatellite Repeats |
| Zdroj: | International Journal of Molecular Sciences Volume 15 Issue 12 Pages 22933-22959 International Journal of Molecular Sciences, Vol 15, Iss 12, Pp 22933-22959 (2014) |
| ISSN: | 1422-0067 |
| Popis: | Passiflora species are distributed throughout Latin America, and Brazil and Colombia serve as the centers of diversity for this genus. We performed cross-species amplification to evaluate 109 microsatellite loci in 14 Passiflora species and estimated the diversity and genetic structure of Passiflora cincinnata, Passiflora setaceae and Passiflora edulis. A total of 127 accessions, including 85 accessions of P. edulis, a commercial species, and 42 accessions of 13 wild species, were examined. The cross-species amplification was effective for obtaining microsatellite loci (average cross-amplification of 70%). The average number of alleles per locus (five) was relatively low, and the average diversity ranged from 0.52 in P. cincinnata to 0.32 in P. setacea. The Bayesian analyses indicated that the P. cincinnata and P. setacea accessions were distributed into two groups, and the P. edulis accessions were distributed into five groups. Private alleles were identified, and suggestions for core collections are presented. Further collections are necessary, and the information generated may be useful for breeding and conservation. |
| Databáze: | OpenAIRE |
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