Regenerative Astaxanthin Extraction from a Single Microalgal (Haematococcus pluvialis) Cell Using a Gold Nano-Scalpel
| Autor: | Tae Soup Shim, Soojeong Cho, Mijeong Kang, Raekeun Gwak, You-Kwan Oh, Bongsoo Kim, Jiye Lee, Kyubock Lee, Ramasamy Praveenkumar |
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| Rok vydání: | 2015 |
| Předmět: |
Haematococcus pluvialis
Microscopy Confocal Materials science biology Cell Extraction (chemistry) Xanthophylls Production efficiency Biorefinery biology.organism_classification Continuous production Nanostructures chemistry.chemical_compound medicine.anatomical_structure chemistry Chlorophyta Astaxanthin Nano medicine General Materials Science Gold Biomedical engineering |
| Zdroj: | ACS Applied Materials & Interfaces. 7:22702-22708 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/acsami.5b07651 |
| Popis: | Milking of microalgae, the process of reusing the biomass for continuous production of target compounds, can strikingly overcome the time and cost constraints associated with biorefinery. This process can significantly improve production efficiency of highly valuable chemicals, for example, astaxanthin (AXT) from Haematococcus pluvialis. Detailed understanding of the biological process of cell survival and AXT reaccumulation after extraction would be of great help for successful milking. Here we report extraction of AXT from a single cell of H. pluvialis through incision of the cell wall by a gold nanoscalpel (Au-NS), which allows single-cell analysis of wound healing and reaccumulation of AXT. Interestingly, upon the Au-NS incision, the cell could reaccumulate AXT at a rate two times faster than the control cells. Efficient extraction as well as minimal cellular damage, keeping cells alive, could be achieved with the optimized shape and dimensions of Au-NS: a well-defined sharp tip, thickness under 300 nm, and 1-3 μm of width. The demonstration of regenerative extraction of AXT at a single cell level hints toward the potential of a milking process for continuous recovery of target compounds from microalgae while keeping the cells alive. |
| Databáze: | OpenAIRE |
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