M2, M3, and M4muscarinic receptors are expressed in the guinea pig gallbladder
| Autor: | Hülya Cabadak, Adolfo E. Cuadra, Beki Kan, Esam E. El-Fakahany |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
medicine.medical_specialty
Contraction (grammar) Guinea Pigs CHO Cells Biology Biochemistry Guinea pig Cricetulus Cricetinae Internal medicine Muscarinic acetylcholine receptor medicine Animals Humans RNA Messenger Receptor Molecular Biology Receptor Muscarinic M3 Receptor Muscarinic M2 Receptor Muscarinic M4 Reverse Transcriptase Polymerase Chain Reaction Gallbladder Receptor Muscarinic M1 Muscarinic acetylcholine receptor M2 Cell Biology Muscarinic acetylcholine receptor M1 Rats Endocrinology Real-time polymerase chain reaction medicine.anatomical_structure |
| Zdroj: | Journal of Receptors and Signal Transduction. 29:63-66 |
| ISSN: | 1532-4281 1079-9893 |
| DOI: | 10.1080/10799890802687416 |
| Popis: | Aim: The identity of muscarinic acetylcholine receptors (mAchR) involved in cholinergic-mediated contraction of the guinea pig gallbladder has been a matter of debate. Different groups have suggested the involvement of M1, M2, M3, or M4 receptor subtypes in the contraction of this tissue. The objective of this study was to identify the mAchR subtypes expressed in the guinea pig gallbladder by RT-PCR. Methods: Total RNA prepared from frozen guinea pig gallbladder tissue was amplified by using specific primers for the M1–M4 receptor subtypes. Results: M2, M3, and M4 transcripts were detected in the following rank order: M4 > M2 > M3. We were unable to demonstrate the expression of the M1 receptor subtype in this tissue. Conclusions: Our results are in agreement with our previous binding and functional data. |
| Databáze: | OpenAIRE |
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