A triticale water-deficit-inducible phytocystatin inhibits endogenous cysteine proteinases in vitro
| Autor: | Ryozo Imai, Magdalena Chojnacka, Joanna Szewińska, Marcin Mielecki, Sławomir Orzechowski, Wiesław Bielawski, Małgorzata Nykiel |
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| Rok vydání: | 2014 |
| Předmět: |
Proteases
Physiology Proteolysis Immunoblotting Plant Science Biology Legumain law.invention chemistry.chemical_compound law Cysteine Proteases Gene Expression Regulation Plant Stress Physiological Complementary DNA medicine Triticum Plant Proteins medicine.diagnostic_test Water Molecular biology Cystatins Droughts Papain Biochemistry chemistry Recombinant DNA biology.protein PMSF Edible Grain Agronomy and Crop Science Cysteine |
| Zdroj: | Journal of plant physiology. 174 |
| ISSN: | 1618-1328 |
| Popis: | Water-deficit is accompanied by an increase in proteolysis. Phytocystatins are plant inhibitors of cysteine proteinases that belong to the papain and legumain family. A cDNA encoding the protein inhibitor TrcC-8 was identified in the vegetative organs of triticale. In response to water-deficit, increases in the mRNA levels of TrcC-8 were observed in leaf and root tissues. Immunoblot analysis indicated that accumulation of the TrcC-8 protein occurred after 72 h of water-deficit in the seedlings. Using recombinant protein, inhibitory activity of TrcC-8 against cysteine proteases from triticale and wheat tissues was analyzed. Under water-deficit conditions, there are increases in cysteine proteinase activities in both plant tissues. The cysteine proteinase activities were inhibited by addition of the recombinant TrcC-8 protein. These results suggest a potential role for the triticale phytocystatin in modulating cysteine proteinase activities during water-deficit conditions. |
| Databáze: | OpenAIRE |
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