Influence of glass and polymer coatings on CHO cell morphology and adhesion
| Autor: | Frederique Kermarec-Marcel, Nicolas Glade, Catherine Pudda, Nathalie Picollet-D'hahan, Fabien Sauter-Starace, Francois Chatelain, Mathias Borella, Stéphanie Garnier-Raveaud, Marc Plissonnier, Thomas Sordel, Franz Bruckert |
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| Rok vydání: | 2007 |
| Předmět: |
Silicon
Materials science Polymers Biophysics chemistry.chemical_element Bioengineering Nanotechnology CHO Cells Substrate (electronics) engineering.material Cell morphology Biomaterials Cricetulus Coated Materials Biocompatible Coating Cricetinae Cell Adhesion Animals Cell adhesion Silicon oxide chemistry.chemical_classification technology industry and agriculture Adhesion Polymer chemistry Chemical engineering Mechanics of Materials Ceramics and Composites engineering Glass |
| Zdroj: | Biomaterials. 28:1572-1584 |
| ISSN: | 0142-9612 |
| DOI: | 10.1016/j.biomaterials.2006.10.032 |
| Popis: | Successful development of cell-on-chip microsystems where living cells are deposited and grown in microfabricated structures is highly dependent on the control of cell/substrate interactions. In this study, several materials of interest were tested for CHO cell growth and morphology: (i) glass, fibronectin-, poly-L-lysine- and 3-aminopropyltriethoxysilane (APTES)--treated glass and UV/O(3)-modified PDMS coating on glass as well as (ii) silicon, poly-L-lysine-, APTES-, O(2) plasma-treated and oxide-coated silicon. In addition, we quantitatively characterized cell adhesion to these substrates using a radial flow detachment assay. Lack of correlation between cell adhesion and cell morphology was systematically observed for all substrates. In particular, we show that PDMS coatings on glass can be finely tuned by UV/O(3) treatment to enhance cell adhesion and induce elongated morphology. Moreover, we observed a low shear stress cell detachment mechanism on silicon oxide coatings on silicon wafers. It is therefore possible with these coatings to selectively influence either cell adhesion or morphology. |
| Databáze: | OpenAIRE |
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