Regulation of alpha 2 integrin gene expression in cells with megakaryocytic features: a common theme of three necessary elements
| Autor: | M M, Zutter, A A, Painter, W D, Staatz, Y L, Tsung |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Integrins
Receptors Collagen Gene Expression Regulation Leukemic Immunology Integrin alpha2 Cell Biology Hematology Burkitt Lymphoma Biochemistry Neoplasm Proteins Enhancer Elements Genetic Genes Antigens CD Leukemia Megakaryoblastic Acute Leukemia Myelogenous Chronic BCR-ABL Positive Tumor Cells Cultured Humans Dimethyl Sulfoxide Megakaryocytes Phorbol 12 13-Dibutyrate |
| Zdroj: | Blood. 86:3006-3014 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v86.8.3006.bloodjournal8683006 |
| Popis: | The alpha 2 beta 1 integrin mediates interactions between cells and the extracellular matrix molecules, collagen and/or laminin. The alpha 2 beta 1 integrin is expressed in a variety of cell types, but in cells of hematopoietic lineage, expression is restricted to megakaryocytes and platelets. Increased expression of the alpha 2 beta 1 integrin during megakaryocytic differentiation is a consequence of transcriptional activation of the alpha 2 gene. We have begun to characterize the role of the 52 flanking region of the alpha 2 integrin gene in regulating expression during megakaryocyte differentiation. A 5-kb fragment of the 52 region directs both cell type and differentiation-dependent expression of a reporter gene in the pluripotent hematopoietic K562 cells upon megakaryocytic differentiation and in the megakaryocytic cell line, Dami. Analysis of a series of 52 deletion mutants indicates that expression of the alpha 2 integrin gene in cells with megakaryocytic features requires a core promoter region, a silencer region, and megakaryocytic enhancers in the distal 52 end. The organization of these three distinct regulatory regions of the alpha 2 promoter/enhancer suggests a common theme for megakaryocytic gene regulation shared with other megakaryocyte-specific proteins, including alpha IIb integrin subunit and platelet factor 4. |
| Databáze: | OpenAIRE |
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