ICR Noncoding RNA Expression Controls Imprinting and DNA Replication at the Dlk1-Dio3 Domain
| Autor: | Satya K. Kota, Ildem Sanli, Christina Begon-Pescia, Tristan Bouschet, Philippe Arnaud, David Llères, Laurent Journot, Michael Girardot, Robert Feil, Alice Marchand, Ryutaro Hirasawa |
|---|---|
| Přispěvatelé: | Institut de Génétique Moléculaire de Montpellier (IGMM), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Génétique, Reproduction et Développement - Clermont Auvergne (GReD), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Clermont Auvergne (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique Moléculaire de Montpellier ( IGMM ), Université de Montpellier ( UM ) -Centre National de la Recherche Scientifique ( CNRS ), Institut de Génomique Fonctionnelle ( IGF ), Centre National de la Recherche Scientifique ( CNRS ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Montpellier 1 ( UM1 ) -Université de Montpellier ( UM ) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
DNA Replication
RNA Untranslated animal diseases Cellular differentiation [SDV.CAN]Life Sciences [q-bio]/Cancer Biology Iodide Peroxidase General Biochemistry Genetics and Molecular Biology Epigenesis Genetic Genomic Imprinting Mice 03 medical and health sciences 0302 clinical medicine [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] Gene expression Animals Epigenetics Molecular Biology Embryonic Stem Cells Locus control region ComputingMilieux_MISCELLANEOUS 030304 developmental biology Genetics 0303 health sciences Replication timing Calcium-Binding Proteins Cell Differentiation [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular biology [SDV.BBM.MN]Life Sciences [q-bio]/Biochemistry Molecular Biology/Molecular Networks [q-bio.MN] Cell Biology DNA Methylation Locus Control Region Non-coding RNA Blastocyst [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry Molecular Biology/Genomics [q-bio.GN] DNA methylation Intercellular Signaling Peptides and Proteins Genomic imprinting 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Cell Developmental Cell, Elsevier, 2014, 31 (1), pp.19-33. ⟨10.1016/j.devcel.2014.08.009⟩ Developmental Cell, Elsevier, 2014, 31 (1), pp.19-33. 〈10.1016/j.devcel.2014.08.009〉 |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2014.08.009⟩ |
| Popis: | SummaryImprinted genes play essential roles in development, and their allelic expression is mediated by imprinting control regions (ICRs). The Dlk1-Dio3 locus is among the few imprinted domains controlled by a paternally methylated ICR. The unmethylated maternal copy activates imprinted expression early in development through an unknown mechanism. We find that in mouse embryonic stem cells (ESCs) and in blastocysts, this function is linked to maternal, bidirectional expression of noncoding RNAs (ncRNAs) from the ICR. Disruption of ICR ncRNA expression in ESCs affected gene expression in cis, led to acquisition of aberrant histone and DNA methylation, delayed replication timing along the domain on the maternal chromosome, and changed its subnuclear localization. The epigenetic alterations persisted during differentiation and affected the neurogenic potential of the stem cells. Our data indicate that monoallelic expression at an ICR of enhancer RNA-like ncRNAs controls imprinted gene expression, epigenetic maintenance processes, and DNA replication in embryonic cells. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|