The expression of placental-type glutathione S-transferase (GST-π) in human cutaneous carcinoma in situ, that is, actinic keratosis and Bowen's disease, compared with normal human skin
| Autor: | Kazuhiro Shimizu, Fumi Toriyama, Hui-min Zhang, Hikotaro Yoshida |
|---|---|
| Rok vydání: | 1995 |
| Předmět: |
Adult
Male Cancer Research Pathology medicine.medical_specialty Skin Neoplasms Placenta Stratum granulosum Bowen's Disease Biology Pregnancy medicine Humans Stratum spinosum Aged Glutathione Transferase Skin Aged 80 and over Bowen's disease Carcinoma in situ Actinic keratosis Keratosis General Medicine Middle Aged medicine.disease Isoenzymes medicine.anatomical_structure Solar keratosis Female Skin Carcinoma Carcinoma in Situ Stratum basale |
| Zdroj: | Carcinogenesis. 16:2327-2330 |
| ISSN: | 1460-2180 0143-3334 |
| DOI: | 10.1093/carcin/16.10.2327 |
| Popis: | The expression of human placental type glutathione S-transferase (GST-pi) was investigated in human cutaneous carcinoma in situ, that is, actinic keratosis and Bowen's disease, and compared with that in normal skin, using Northern blot and immunohistochemical analysis. In Northern blot examination, the expression of GST-pi transcript was recognized in all instances. Carcinoma in situ showed significantly higher expressions of GST-pi than normal skin. In the immunohistochemical examination, nuclear staining of GST-pi was noticed in some dysplastic cells of carcinoma in situ, especially in Bowen's disease. In actinic keratosis, a framework appearance was noticed in the staining pattern at a lower magnification because the lower part of the cytoplasm of dysplastic cells lining the stratum basale was positive for GST-pi, and all cells of the stratum granulosum and more cells of the stratum spinosum showed stronger GST-pi positive reaction than normal skin. In Bowen's disease, GST-pi positive, dysplastic cells existed throughout the epidermis. Because GST-pi positive, dysplastic cells and GST-pi positive, normal looking squamous cells made the GST-pi positive cell nests throughout the epidermis, and GST-pi positive, dysplastic cells, and GST-pi negative, normal looking cells coexisted in the parabasal layer, they showed a sawtooth appearance in the staining pattern at a lower magnification. These findings suggest that GST-pi is involved in the process of carcinogenesis. |
| Databáze: | OpenAIRE |
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