Volatile Anesthetics Rapidly Increase Dendritic Spine Density in the Rat Medial Prefrontal Cortex during Synaptogenesis
| Autor: | Dominique Muller, Laszlo Vutskits, Adrian Briner, Mathias De Roo, Walid Habre, Alexandre Dayer |
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| Rok vydání: | 2010 |
| Předmět: |
Dendritic spine
Synaptogenesis Prefrontal Cortex/ cytology/drug effects/growth & development 0302 clinical medicine Cell Death/drug effects 030202 anesthesiology Isoflurane/analogs & derivatives/pharmacology Organic Chemicals Coloring Agents Prefrontal cortex Cell Death Isoflurane Pyramidal Cells Iontophoresis Fluoresceins Immunohistochemistry medicine.anatomical_structure Cytoarchitecture Cerebral cortex Anesthetics Inhalation Dendritic Spines/ drug effects medicine.drug Methyl Ethers medicine.medical_specialty Dendritic Spines Prefrontal Cortex Biology Anesthetics Inhalation/ pharmacology Methyl Ethers/pharmacology Article Sevoflurane 03 medical and health sciences Internal medicine In Situ Nick-End Labeling medicine Animals Rats Long-Evans Rats Wistar Synapses/ drug effects Pyramidal Cells/drug effects/ultrastructure ddc:616.8 Rats Anesthesiology and Pain Medicine Endocrinology Synapses Anesthetic Desflurane Neuroscience 030217 neurology & neurosurgery |
| Zdroj: | Anesthesiology Anesthesiology, Vol. 112, No 3 (2010) pp. 546-556 |
| ISSN: | 0003-3022 |
| DOI: | 10.1097/aln.0b013e3181cd7942 |
| Popis: | Background Recent experimental observations suggest that, in addition to induce neuroapoptosis, anesthetics can also interfere with synaptogenesis during brain development. The aim of this study was to pursue this issue by evaluating the exposure time-dependent effects of volatile anesthetics on neuronal cytoarchitecture in 16-day-old rats, a developmental stage characterized by intense synaptogenesis in the cerebral cortex. Methods Whistar rats underwent isoflurane (1.5%), sevoflurane (2.5%), or desflurane (7%) anesthesia for 30, 60, and 120 min at postnatal day 16, and the effect of these treatments on neuronal cytoarchitecture was evaluated 6 h after the initiation of anesthesia. Cell death was assessed using Fluoro-Jade B staining and terminal deoxynucleotidyl transferase deoxyuridine triphosphate nick-end labeling assay. Ionotophoretic injections into layer 5 pyramidal neurons in the medial prefrontal cortex allowed visualization of dendritic arbor. Tracing of dendritic tree was carried out using the Neurolucida station (Microbrightfield, Williston, VT), whereas dendritic spines were analyzed using confocal microscopy. Results Up to a 2-h-long exposure, none of the volatile drugs induced neuronal cell death or significant changes in gross dendritic arbor pattern of layer 5 pyramidal neurons in pups at postnatal day 16. In contrast, these drugs significantly increased dendritic spine density on dendritic shafts of these cells. Importantly, considerable differences were found between these three volatile agents in terms of exposure time-dependent effects on dendritic spine density. Conclusion These new results suggest that volatile anesthetics, with different potencies and without inducing cell death, could rapidly interfere with physiologic patterns of synaptogenesis and thus might impair appropriate circuit assembly in the developing cerebral cortex. |
| Databáze: | OpenAIRE |
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