Overexpression of zmm28 increases maize grain yield in the field
| Autor: | Olga N. Danilevskaya, Ben P. Weers, Keith Roesler, Zhenglin Hou, Wengang Zhou, Xin Meng, Hua Mo, Renee Lafitte, Jingrui Wu, Carl Walker, Shai J. Lawit, Jeffrey E. Habben, Justin Jantes, John L. Van Hemert, Joanne Hunt, Bo Shen, Joshua Clapp, Matthew Wimmer, Layton Peddicord, Melo Rosana, Nick Mongar, Libby Trecker, Mary Rupe, Lu Liu, Jill Thomas, Yiwei Wang, Jeffrey R. Schussler, Jindong Sun, Kristin Haug Collet |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Crops
Agricultural 0106 biological sciences 0301 basic medicine Germplasm Plant growth Nitrogen Plant Biology Genetically modified crops Biology Genes Plant Photosynthesis Nitrate Reductase Zea mays 01 natural sciences carbon assimilation maize yield 03 medical and health sciences Carbon assimilation Glutamate-Ammonia Ligase Amino Acid Sequence Plant breeding nitrogen utilization MADS-box Plant Proteins Multidisciplinary Biological Sciences Plants Genetically Modified zmm28 Plant Leaves 030104 developmental biology PNAS Plus Agronomy Grain yield Edible Grain Transcriptome Protein Binding 010606 plant biology & botany |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America |
| ISSN: | 1091-6490 0027-8424 |
| DOI: | 10.1073/pnas.1902593116 |
| Popis: | Significance In the approaching decades, food security will likely be more of an issue as there will be an increased demand for grain which will need to be met in an environmentally sustainable manner. To date, commercial transgenic maize has primarily targeted resistance to insects and herbicides. Here we describe a transgenic approach to improve the yield and yield stability of maize. We have demonstrated that increasing and extending the expression of a maize gene, zmm28, alters vegetative and reproductive growth parameters and significantly enhances yield in large-scale field trials conducted over multiple years. We conclude that alteration in expression of a native maize gene in maize can create a substantially positive change in a complex trait like grain yield. Increasing maize grain yield has been a major focus of both plant breeding and genetic engineering to meet the global demand for food, feed, and industrial uses. We report that increasing and extending expression of a maize MADS-box transcription factor gene, zmm28, under the control of a moderate-constitutive maize promoter, results in maize plants with increased plant growth, photosynthesis capacity, and nitrogen utilization. Molecular and biochemical characterization of zmm28 transgenic plants demonstrated that their enhanced agronomic traits are associated with elevated plant carbon assimilation, nitrogen utilization, and plant growth. Overall, these positive attributes are associated with a significant increase in grain yield relative to wild-type controls that is consistent across years, environments, and elite germplasm backgrounds. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|