Quantitation by myeloperoxidase assay of neutrophil accumulation at the site ofin vivo allergic reactions

Autor:	Pierre-Michel Bedard, Paul C. Atkins, Burton Zweiman
Rok vydání:	1983
Předmět:	biology Neutrophils Sonication Immunology Cell Count Human skin Dermatitis Atopic Standard curve chemistry.chemical_compound Linear relationship Peroxidases chemistry Biochemistry In vivo Myeloperoxidase biology.protein Humans Immunology and Allergy Histamine Peroxidase
Zdroj:	Journal of Clinical Immunology. 3:84-89
ISSN:	1573-2592 0271-9142
DOI:	10.1007/bf00919143
Popis:	Skin window techniques to investigate neutrophil inflammatory reactions in human skin have been limited by cellular distortion and difficulties in quantitation. We have developed a quantitative approach based on the assessment of the myeloperoxidase (MPO) released from sonicated membrane filters to which exuding inflammative cells had adhered. Our studies have shown that (1) a standard curve can be derived for each subject from the linear relationship between amounts of MPO released and designated numbers of blood neutrophils which have been aspirated onto such filters; (2) the number of neutrophils adhering to filters appended to skin window sites can then be reliably estimated by comparing the amount of MPO released by sonication of such filters with the standard curve; and (3) neutrophil accumulation is greater at pollen-challenged than control sites in sensitive subjects.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::c4cc51a0c64419aec3e85aadf00be216 https://doi.org/10.1007/bf00919143 Zobrazit plný text záznamu Full text from SpringerLink