Cytokine production in synovial tissue of mice with collagen-induced arthritis (CIA)
| Autor: | Lars Klareskog, M J Litton, Eva Lindroos, A Müssener |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Male
musculoskeletal diseases Pathology medicine.medical_specialty Necrosis medicine.medical_treatment Immunology Pannus Arthritis Tarsal Joints Mice Antigen Antigens CD Transforming Growth Factor beta medicine Animals Immunology and Allergy business.industry Monokines Synovial Membrane Original Articles medicine.disease Arthritis Experimental Monokine Kinetics Cytokine medicine.anatomical_structure Mice Inbred DBA Organ Specificity Rheumatoid arthritis Cytokines Collagen medicine.symptom Synovial membrane business Ankle Joint Biomarkers |
| Zdroj: | Clinical and Experimental Immunology. 107:485-493 |
| ISSN: | 1365-2249 0009-9104 |
| DOI: | 10.1046/j.1365-2249.1997.3181214.x |
| Popis: | SUMMARY The kinetics of cytokine production in arthritic limbs of mice with CIA was determined by using modified immunohistochemical techniques. Tissue cryostat sections of undecalcified whole paws were analysed for the presence of tumour necrosis factor-alpha (TNF-α), IL-6, IL-2, IL-4, IL-5 interferon-gamma (IFN-γ), transforming growth factor-beta 2 (TGF-β2) and TGF-β3. Locally produced TNF-α, IL-6 and TGF-β2 were observed within the lining layer, sublining and pannus at all stages of disease. The staining of TNF-α was particularly intense at the cartilage–pannus junction. In contrast to the monokines, IFN-γ and TGF-β3 were only expressed in scattered cells within the deeper layers of the synovia. Interestingly, IFN-γ was not present in the late phase of CIA, despite the continued presence of TNF-α and IL-6 in the pannus. Production of IL-2, IL-4 or IL-5 was not detected in any joint. The observed pattern of a relative paucity of T cell-derived cytokines and an abundance of monokines during the late phase of T cell-dependent CIA indicates that the synovial cytokine pattern previously described in rheumatoid arthritis (RA) is fully compatible with a pathogenic role of T cells. The temporal as well as spatial dissociation between expression of T cell-derived cytokines and monokines indicates that T cell-independent mechanisms may also be of importance in the triggering of monokine production during arthritis. |
| Databáze: | OpenAIRE |
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