Phosphorylated proteomics analysis of human coronary artery endothelial cells stimulated by Kawasaki disease patients serum

Autor: Shui-Ming Li, Wan-Ting Liu, Qi-Jian Yi, Fang Yang, Hong-Ling Jia, Shuai Zhang
Rok vydání: 2018
Předmět:
MAPK/ERK pathway
Male
Proteomics
lcsh:Diseases of the circulatory (Cardiovascular) system
Spectrometry
Mass
Electrospray Ionization
Hub proteins
Network analyzer analysis
030204 cardiovascular system & hematology
Mucocutaneous Lymph Node Syndrome
Pathogenesis
03 medical and health sciences
0302 clinical medicine
Tandem Mass Spectrometry
Medicine
Humans
030212 general & internal medicine
Protein Interaction Maps
Phosphorylation
PI3K/AKT/mTOR pathway
Cells
Cultured
Chromatography
High Pressure Liquid
Chromatography
Reverse-Phase
biology
business.industry
KD
Endothelial Cells
Infant
Proteins
Angiopoietin receptor
Coronary Vessels
Phosphorylated Peptide
Blot
lcsh:RC666-701
Case-Control Studies
Child
Preschool
Phosphorylated proteomics
Cancer research
biology.protein
Female
HCAECs
Cardiology and Cardiovascular Medicine
business
Signal Transduction
Research Article
Zdroj: BMC Cardiovascular Disorders
BMC Cardiovascular Disorders, Vol 19, Iss 1, Pp 1-9 (2019)
ISSN: 1471-2261
Popis: Background Kawasaki disease (KD) is an acute febrile childhood systemic vasculitis that disturbs coronary arteries. The pathogenesis remains unknown. The study of phosphorylated proteins helps to elucidate the relevant pathophysiological mechanisms of cardiovascular disease. However, few researches explored phosphorylated proteins in KD patients. Methods We compared phosphoprotein profiles of HCAECs stimulated by the serum of KD patients and normal children using iTRAQ technology, TiO2 enrichment phosphorylated peptide and MS analysis. Then we conducted the functional analysis by ClueGO and the biological interaction networking analysis by ReactomeFIViz. Western blotting was performed to identify the hub proteins. Results Our results revealed that phosphorylation of 148 proteins showed different intensities between the two HCAECs groups, which are enriched in MAPK, VEGFR, EGFR, Angiopoietin receptor, mTOR, FAK signaling pathway and so on. Through the Network Analyzer analysis, the hub proteins are CDKN1A, MAPK1 and POLR2A, which were experimentally validated. Conclusion In summary, we provided evidence addressing the valuable phosphorylation signaling that could be useful resource to understand the molecular mechanism and the potential targets for novel therapy of KD. Electronic supplementary material The online version of this article (10.1186/s12872-018-0982-2) contains supplementary material, which is available to authorized users.
Databáze: OpenAIRE
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