Intranasal delivery of naked DNA encoding the LACK antigen leads to protective immunity against visceral leishmaniasis in mice
| Autor: | Vicente Larraga, Ulisses Gazos Lopes, Wallace Pacienza Lima, Luiz Dione Barbosa de Melo, Bartira Rossi-Bergmann, Daniel Cláudio de Oliveira Gomes, Eduardo Fonseca Pinto |
|---|---|
| Přispěvatelé: | De Melo, Luiz Dione Barbosa [0000-0003-2445-9943], Lima, Wallace Pacienza [0000-0002-5489-2206], Larraga, Vicente [0000-0003-1260-7400], Lopes, Ulisses Gazos [0000-0002-0216-3482], Rossi-Bergmann, Bartira [0000-0002-3791-8996], De Melo, Luiz Dione Barbosa, Lima, Wallace Pacienza, Larraga, Vicente, Lopes, Ulisses Gazos, Rossi-Bergmann, Bartira |
| Rok vydání: | 2006 |
| Předmět: |
Protozoan Vaccines
Protozoan Proteins Leishmaniasis Cutaneous Spleen Antigens Protozoan Biology Interferon-gamma Mice Cutaneous leishmaniasis LACK medicine Animals Leishmania infantum Administration Intranasal Visceral leishmaniasis Mice Inbred BALB C General Veterinary General Immunology and Microbiology Leishmania chagasi Public Health Environmental and Occupational Health Leishmaniasis DNA DNA Protozoan medicine.disease biology.organism_classification Virology Vaccination Infectious Diseases medicine.anatomical_structure Treatment Outcome Intranasal Liver Naked DNA Immunology Molecular Medicine Leishmaniasis Visceral Interleukin-4 Vaccine Plasmids |
| Zdroj: | Digital.CSIC. Repositorio Institucional del CSIC instname |
| ISSN: | 0264-410X |
| Popis: | 5 p.-3 fig. We previously showed that intranasal (i.n.) vaccination with pCIneo plasmid encoding the leishmanial LACK gene (pCIneo-LACK) induces long-lasting protective immunity against cutaneous leishmaniasis in mice. In this work, we proposed to investigate whether the efficacy of i.n. pCIneo-LACK is extensive to visceral leishmaniasis. BALB/c mice received two i.n. doses of 30 μg pCIneo-LACK prior to intravenous (i.v.) infection with Leishmania chagasi. Vaccinated mice developed significantly lower parasite burden in the liver and spleen than control mice receiving empty pCIneo or saline. The spleen cells of vaccinated mice produced significantly increased IFN-γ and IL-4 concomitant with decreased IL-10 production during infection. Serum levels of specific IgG were elevated whereas TNF-α were decreased as compared with controls. These results show that the practical needle-free i.n. pCIneo-LACK vaccine displays potential broad-spectrum activity against leishmaniasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect