Variability of core microbiota in newly diagnosed treatment-naïve paediatric Inflammatory Bowel Disease patients
| Autor: | Anat Eck, C. M. F. Kneepkens, Carel F.W. Peeters, Andries E. Budding, T. G. J. de Meij, E. F. J. de Groot, N. K. H. de Boer, Ad A. van Bodegraven, Marc A. Benninga, Paul H. M. Savelkoul |
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| Přispěvatelé: | AGEM - Digestive immunity, AGEM - Re-generation and cancer of the digestive system, ARD - Amsterdam Reproduction and Development, Paediatric Gastroenterology, RS: NUTRIM - R2 - Liver and digestive health, Med Microbiol, Infect Dis & Infect Prev, MUMC+: DA Medische Microbiologie en Infectieziekten (5), Pediatric surgery, Amsterdam Reproduction & Development (AR&D), Gastroenterology and hepatology, APH - Methodology, Epidemiology and Data Science, AII - Inflammatory diseases, Medical Microbiology and Infection Prevention |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine PATHOGENESIS Individuality lcsh:Medicine Colonoscopy Crohn's Disease CHILDREN Disease Gut flora Pediatrics Gastroenterology Inflammatory bowel disease 0302 clinical medicine Medicine and Health Sciences Child lcsh:Science Prospective cohort study Crohn's disease Multidisciplinary Ecology medicine.diagnostic_test biology GUT MICROBIOTA Genomics Colitis Ulcerative colitis CROHNS-DISEASE Alistipes putredinis Shannon Index Medical Microbiology Child Preschool Female 030211 gastroenterology & hepatology Anatomy Research Article medicine.medical_specialty Ecological Metrics Adolescent Immunology IMPROVEMENT Microbial Genomics Gastroenterology and Hepatology ENTERAL NUTRITION Microbiology Autoimmune Diseases 03 medical and health sciences INTESTINAL MICROBIOTA Internal medicine Genetics medicine Ulcerative Colitis Humans MODULATION Alistipes RISING INCIDENCE Bacteria business.industry Inflammatory Bowel Disease Ecology and Environmental Sciences lcsh:R Organisms Case-control study Biology and Life Sciences Species Diversity Inflammatory Bowel Diseases biology.organism_classification medicine.disease digestive system diseases Gastrointestinal Microbiome Gastrointestinal Tract 030104 developmental biology Case-Control Studies Etiology FECAL MICROBIOTA Clinical Immunology lcsh:Q Microbiome Clinical Medicine business Digestive System |
| Zdroj: | PLoS ONE, 13(8). Public Library of Science PLoS ONE, Vol 13, Iss 8, p e0197649 (2018) PLOS ONE, 13(8):0197649. Public Library of Science PLoS ONE, 13(8):e0197649. Public Library of Science PLoS ONE de Meij, T G J, de Groot, E F J, Peeters, C F W, de Boer, N K H, Kneepkens, C M F, Eck, A, Benninga, M A, Savelkoul, P H M, van Bodegraven, A A & Budding, A E 2018, ' Variability of core microbiota in newly diagnosed treatment-naïve paediatric inflammatory bowel disease patients ', PLoS ONE, vol. 13, no. 8, e0197649 . https://doi.org/10.1371/journal.pone.0197649 |
| ISSN: | 1932-6203 |
| DOI: | 10.1101/317263 |
| Popis: | BACKGROUND & AIMSIntestinal microbiota is considered to play a crucial role in the aetiology of inflammatory bowel disease (IBD). We aimed to describe faecal microbiota composition and dynamics in a large cohort of children with de novo (naïve) IBD, in comparison to healthy paediatric controls (HC).METHODSIn this prospective study, performed at two tertiary centres, faecal samples from newly diagnosed, treatment-naïve paediatric IBD patients were collected prior to bowel cleansing for colonoscopy (t0) and 1, 3 and 6 weeks and 3 months after initiation of therapy. The microbial profiles of Crohn’s disease (CD) and Ulcerative colitis (UC) patients were compared with HC and linked to therapeutic response. Microbiota composition was analysed by IS-pro technology.RESULTSMicrobial profiles of 104 new IBD-patients (63 CD, 41 UC, median age 14.0 years) were compared to 61 HC (median 7.8 years). IBD was mainly characterised by decreased abundance of Alistipes finegoldi and Alistipes putredinis, which characterize a healthy state microbial core. The classifier including these core species as predictors achieved an AUC of the ROC curve of .87. Core bacteria tended to regain abundance during treatment, but did not reach healthy levels.CONCLUSIONFaecal microbiota profiles of children with de novo CD and UC can be discriminated from HC with high accuracy, mainly driven by a decreased abundance of species shaping the microbial core in the healthy state. Paediatric IBD can therefore be characterized by decreased abundance of certain bacterial species reflecting the healthy state rather than by the introduction of pathogens. |
| Databáze: | OpenAIRE |
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