Presence of occult cytomegalovirus infection in the brain after orthotopic liver transplantation. An autopsy study of 83 cases
| Autor: | Rosa Miquel, Antonio Cardesa, Xavier Claramonte, Jaume Muntané, Pedro Jares, Teresa Ribalta, Julio A. Martinez |
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| Rok vydání: | 2002 |
| Předmět: |
Human cytomegalovirus
Adult Male Pathology medicine.medical_specialty Adolescent Population Congenital cytomegalovirus infection Cytomegalovirus medicine.disease_cause Polymerase Chain Reaction Herpesviridae Pathology and Forensic Medicine Central nervous system disease Immunocompromised Host Postoperative Complications Betaherpesvirinae medicine Humans Encephalitis Viral education Child Molecular Biology In Situ Hybridization Aged DNA Primers Retrospective Studies education.field_of_study biology Cell Biology General Medicine Middle Aged medicine.disease biology.organism_classification Liver Transplantation Transplantation Cytomegalovirus Infections DNA Viral Female Nested polymerase chain reaction |
| Zdroj: | Virchows Archiv : an international journal of pathology. 440(2) |
| ISSN: | 0945-6317 |
| Popis: | Cytomegalovirus (CMV) infection remains a highly prevalent systemic complication following orthotopic liver transplantation (LT), accounting for a significant increase in morbidity and affiliated costs. However, unlike other immunosuppressed groups of population, CMV infection of the central nervous system in LT is rarely diagnosed, either clinically or postmortem. Furthermore, in 20% of the LT patients who develop preterminal neurological complications, the etiology remains undetermined. With the hypothesis that at least some of these cases could be related to an occult CMV infection, we examined brain tissue from 83 unselected autopsies of LT patients by morphological, immunohistochemical (IHC), in situ hybridization (ISH), and nested polymerase chain reaction (nested PCR) techniques. Microglial nodules were observed in 17 brains of the LT group (20.4%) but in none of the 36 controls. Isolated positive cells by either IHC, ISH, or both techniques, were identified in 11 LT patients (13.2%) and in 2 controls (5.5%). CMV DNA amplification was obtained from paraffin-embedded tissues in 41 of 81 LT cases (50.6%), and in 5 controls (13.8%) (P=0.00017). Viral inclusion bodies, inflammatory infiltrates, or necrotizing changes were not identified in any case. Our findings indicate an increased susceptibility of the brain of LT patients to occult infection by CMV and suggest that a latent or low-grade infection of the central nervous system could operate as a reservoir of the CMV and play a role in some of the unexplained neurological symptoms that appear in the postoperative period. |
| Databáze: | OpenAIRE |
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