Characterization of Indole-Negative Bacteroides fragilis Group Species with Use of Polymerase Chain Reaction Fingerprinting and Resistance Profiles
| Autor: | Th. Montag, Ellie J. C. Goldstein, Arne C. Rodloff, B. Hampel, S. Hunt Gerardo, Diane M. Citron, G. Schönian, M. C. Claros |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Bacterial Microbiology (medical) Indoles Polymerase Chain Reaction law.invention Microbiology Bacteroides fragilis Species Specificity law Ampicillin medicine Bacteroides Humans Bacteroidaceae Polymerase chain reaction DNA Primers Molecular Epidemiology Base Sequence biology Drug Resistance Microbial Bacteroides Infections biology.organism_classification DNA Fingerprinting Bacterial Typing Techniques Infectious Diseases Minisatellite Microsatellite Primer (molecular biology) medicine.drug |
| Zdroj: | Clinical Infectious Diseases. 23:S66-S72 |
| ISSN: | 1537-6591 1058-4838 |
| DOI: | 10.1093/clinids/23.supplement_1.s66 |
| Popis: | Biochemical tests alone do not adequately differentiate the various Bacteroides species, groups, and antimicrobial-resistant variants. Consequently, we used a polymerase chain reaction (PCR) fingerprinting technique, with either a single nonspecific primer derived from the t-DNA intergenic spacer region (T3B) or a single primer that anneals to minisatellite and microsatellite DNA sequences (M13 core), to identify and characterize 58 clinical isolates of Bacteroides fragilis group species (B. fragilis, B. distasonis, and B. caccae). In addition to species- and subspecies-specific differences, 4 strains of B. fragilis, 1 of B. distasonis, and 3 of B. caccae that showed increased resistance to imipenem, ampicillin, and ampicillin/sulbactam also produced unique PCR fingerprint profiles. Analysis by the clinical source of isolation (i.e., blood or intraabdominal, skin, or soft-tissue infection) indicated that no particular PCR fingerprint type was associated with greater pathogenicity or any individual clinical source. The PCR fingerprinting technique proves to be a useful tool for species identification and taxonomic studies, as well as for epidemiological studies of Bacteroides species. |
| Databáze: | OpenAIRE |
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