Bcl-xL expression after contusion to the rat spinal cord
| Autor: | Ziming Ye, J. Regino Perez-Polo, David J. McAdoo, Jingxin Qiu, Clarie E. Hulsebosch, Guo Ying Xu, Karin N. Westlund, Olivera Nesic, Harriet C. Rea |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Spinothalamic tract Spinothalamic Tracts Contusions Blotting Western bcl-X Protein Pain Bcl-xL Apoptosis DNA Fragmentation Central nervous system disease Histones Rats Sprague-Dawley Proto-Oncogene Proteins Medicine Animals Spinal cord injury Spinal Cord Injuries bcl-2-Associated X Protein Microscopy Confocal biology business.industry DNA Spinal cord medicine.disease Pathophysiology Rats medicine.anatomical_structure Proto-Oncogene Proteins c-bcl-2 Spinal Cord Fluorescent Antibody Technique Direct Cancer research biology.protein DNA fragmentation Neurology (clinical) business Neuroscience Locomotion |
| Zdroj: | Journal of neurotrauma. 18(11) |
| ISSN: | 0897-7151 |
| Popis: | After contusion-derived spinal cord injury, (SCI) there is localized tissue disruption and energy failure that results in early necrosis and delayed apoptosis, events that contribute to chronic central pain in a majority of patients. We assessed the extent of contusion-induced apoptosis of neurons in a known central pain-signaling pathway, the spinothalamic tract (STT), which may be a contributor to SCI-induced pain. We observed the loss of STT cells and localized increase of DNA fragmentation and cytoplasmic histone-DNA complexes, which suggested potential apoptotic changes among STT neurons after SCI. We also showed SCI-associated changes in the expression of the antiapoptotic protein Bcl-xL, especially among STT cells, consistent with the hypothesis that Bcl-xL regulates the extent of apoptosis after SCI. Apoptosis in the injured spinal cord correlated well with prompt decreases in Bcl-xL protein levels and Bcl-xL/Bax protein ratios at the contusion site. We interpret these results as evidence that regulation of Bcl-xL may play a role in neural sparing after spinal injury and pain-signaling function. |
| Databáze: | OpenAIRE |
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