Modifying the proliferative state of target cells to control DNA expression and identifying cell types transfected in vivo
| Autor: | Kristi S. Anseth, Charles Y. Cheung, David J. Mooney, Hyunjoon Kong, J. Kent Leach, Kathryn W. Riddle, Claudia Fischbach |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Cell type medicine.medical_treatment Cell Basic fibroblast growth factor Green Fluorescent Proteins Gene Expression 02 engineering and technology Biology Transfection 03 medical and health sciences chemistry.chemical_compound Mice Plasmid Cell Movement Drug Discovery medicine Genetics Animals Molecular Biology 030304 developmental biology Cell Proliferation Pharmacology 0303 health sciences Skull Fractures Cell growth Hepatocyte Growth Factor Growth factor 021001 nanoscience & nanotechnology Molecular biology Rats medicine.anatomical_structure chemistry Rats Inbred Lew Bone Morphogenetic Proteins NIH 3T3 Cells Molecular Medicine Hepatocyte growth factor Fibroblast Growth Factor 2 0210 nano-technology medicine.drug Plasmids |
| Zdroj: | Molecular therapy : the journal of the American Society of Gene Therapy. 15(2) |
| ISSN: | 1525-0016 |
| Popis: | Although the majority of current gene transfer techniques have focused on increasing the ability of the DNA to enter the cell, it is possible that changing the proliferative and migratory state of cells will influence the cells ability to take up and express plasmid DNA. This study was designed to test the hypothesis that growth factors (basic fibroblast growth factor (bFGF) and hepatocyte growth factor/scatter factor (HGF/SF)) used to alter the proliferative and migratory state of cells can alter plasmid DNA uptake and expression. In vitro studies indicate that enhancing cell proliferation with growth factor exposure enhances plasmid DNA uptake and expression. Furthermore, dual localized delivery of bFGF and plasmid DNA in vivo increases the expression, 3–6 times over control, as compared to plasmid delivery alone. Dual delivery of a factor promoting cell proliferation and a plasmid led to a further increase in the expression of the plasmid encoding bone morphogenetic protein-2 in a rat cranial defect by specific cell populations. The results of these studies suggest that increasing the proliferative state of target cell populations can enhance non-viral gene transfer. |
| Databáze: | OpenAIRE |
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