Detection of C-reactive protein based on magnetic nanoparticles and capillary zone electrophoresis with laser-induced fluorescence detection
| Autor: | Mei Yu Su, Jian Ying Yang, Wen Jie Li, Ting Yu Shu, Yi Jyun Lin, Mine-Yine Liu, Ting-Yu Lin, Yen Yi Chen |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Analytical chemistry
Biochemistry Analytical Chemistry Capillary electrophoresis Limit of Detection Humans Particle Size Bovine serum albumin Magnetite Nanoparticles Laser-induced fluorescence Chromatography biology Chemistry Osmolar Concentration Organic Chemistry Electrophoresis Capillary Reproducibility of Results General Medicine Hydrogen-Ion Concentration Fluorescence C-Reactive Protein Spectrometry Fluorescence Linear range Ionic strength Linear Models biology.protein Magnetic nanoparticles Quantitative analysis (chemistry) |
| Zdroj: | Journal of Chromatography A. 1315:188-194 |
| ISSN: | 0021-9673 |
| DOI: | 10.1016/j.chroma.2013.09.042 |
| Popis: | A simple and fast method based on magnetic nanoparticles (MNPs) and capillary zone electrophoresis (CZE) with laser-induced fluorescence (LIF) detection was developed for the detection of C-reactive protein (CRP). To optimize the CZE conditions, several factors including buffer compositions, buffer ionic strength, buffer pH, applied voltage and capillary temperature have been examined. The optimal separation buffer selected was a 30 mM sodium phosphate (PB) buffer, pH 8.0. The optimal CE applied voltage and temperature selected were 20 kV and 35 °C, respectively. The CZE profile of the MNP-1°Ab-CRP-2°Ab/FITC bioconjugates showed good reproducibility. One major peak was observed for the MNP bioconjugates. The quantitative analysis also showed good results. The coefficient of variation (CV%) for the major peak area was 8.7%, and the CV% for the major peak migration time was 2.5%. The linear range for CRP analysis was 10–150 μg/mL, and the concentration limit of detection (LOD) was 9.2 μg/mL. Non-specific interactions between bovine serum albumin (BSA) and the system can be prevented by including 10% (v/v) of human plasma in the binding buffers. The CE/LIF method might be helpful for analyzing high concentrations of CRP in a patient's plasma after an acute-phase inflammation. This new method demonstrated the possibility of using MNPs and CE/LIF for the detection of proteins, and provided information for the establishment of appropriate CE conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect