The protective effect of a novel antioxidant gene from Mycobacterium avium against nitrosative and oxidative stress in E. coli
| Autor: | Xueping Ning, Min Ling, Shiyi He, Dongjun Yang, Aiyan Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology Sequence Homology Oxidative phosphorylation medicine.disease_cause Applied Microbiology and Biotechnology Antioxidants Microbiology Mycobacterium tuberculosis 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Escherichia coli medicine Cloning Molecular Sodium nitrite Pathogen Base Sequence Sodium Nitrite biology Gene Expression Regulation Bacterial Hydrogen Peroxide General Medicine biology.organism_classification Oxidative Stress 030104 developmental biology chemistry Genes Bacterial Nitrosative Stress S-Nitrosoglutathione Nontuberculous mycobacteria Reactive Oxygen Species Oxidative stress Bacteria Mycobacterium avium Biotechnology Mycobacterium |
| Zdroj: | World Journal of Microbiology and Biotechnology. 33 |
| ISSN: | 1573-0972 0959-3993 |
| DOI: | 10.1007/s11274-017-2294-6 |
| Popis: | The production of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI) is an important host defense mechanism in response to infection by Mycobacterium tuberculosis. A variety of genes have been implicated in resistance to ROI and RNI, including noxR1. However, studies in Mycobacterium avium, an important pathogen among nontuberculous mycobacteria, are limited. We aim to investigate the role of a novel gene cloned from M. avium with high similarity to noxR1, noA, in resistance against RNI and ROI in M. tuberculosis. After subcloning noA into vector for expression in E. coli, we performed survival rate analysis in the bacteria transformed with noA (pET-noA) and without noA (pET-his) after exposure to nitrosative stresses by S-nitrosoglutathione (GSNO) and sodium nitrite, and oxidative stresses by H2O2. Compared with pET-his, the survival rate of pET-noA was 1 log10-fold higher after exposure to GSNO and sodium nitrite. We observed 1 log10-fold, 2 log10-fold and 3 log10-fold higher survival rate in pET-noA than pET-his after exposure to H2O2 for 3, 6 and 9 h, respectively. With the combined treatment of H2O2 and GSNO, we found more than 2 log10-fold increase in survival rate in pET-noA comparing with pET-his, suggesting a possible synergistic effect. In summary, noA gene cloned from M. avium has been shown to protect E. coli from both RNI and ROI. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink