Anti-Influenza A Virus Activities of Type I/III Interferons-Induced Mx1 GTPases from Different Mammalian Species
| Autor: | Phai Dam Van, Daniel Desmecht, Dao Bui Tran Anh, Anne-Sophie Van Laere, Mutien-Marie Garigliany |
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| Rok vydání: | 2019 |
| Předmět: |
Myxovirus Resistance Proteins
Buffaloes Swine Immunology Defence mechanisms GTPase Biology medicine.disease_cause Interferon Lambda 03 medical and health sciences Dogs Virology Influenza A virus medicine Animals Humans Horses 030304 developmental biology 0303 health sciences Effector Schmallenberg virus 04 agricultural and veterinary sciences Cell Biology biology.organism_classification HEK293 Cells Interferon Type I Mx protein 040102 fisheries 0401 agriculture forestry and fisheries Cattle Interferons Intracellular |
| Zdroj: | Journal of Interferon & Cytokine Research. 39:274-282 |
| ISSN: | 1557-7465 1079-9907 |
| DOI: | 10.1089/jir.2018.0157 |
| Popis: | Type I/III interferons provide powerful and universal innate intracellular defense mechanisms against viruses. Among the antiviral effectors induced, Mx proteins of some species appear as key components of defense against influenza A viruses. It is expected that such an antiviral protein must display a platform dedicated to the recognition of said viruses. In an attempt to identify such platform in human MxA, an evolution-guided approach capitalizing on the antagonistic arms race between MxA and its viral targets and the genomic signature it left on primate genomes revealed that the surface-exposed so-called "loop L4", which protrudes from the compact structure of the MxA stalk, is a hotspot of recurrent positive selection. Since MxA is archetypic of Mx1 proteins in general, we reasoned that the L4 loop also functions as a recognition platform for influenza viruses in the Mx1 proteins of other species that had been exposed to the virus for ever. In this study, the anti-influenza activity of 5 distinct mammalian Mx1 proteins was measured by comparing the number of viral nucleoprotein-positive cells 7 h after infection in a sample of 100,000 cells expected to contain both Mx1-positive and Mx1-negative cell subpopulations. The systematic depletion ( |
| Databáze: | OpenAIRE |
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