Wading pool water contaminated with both noroviruses and astroviruses as the source of a gastroenteritis outbreak
Autor: | C.-H. von Bonsdorff, Leena Maunula, A Pönka, S Kalso |
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Rok vydání: | 2004 |
Předmět: |
Adult
Male Adolescent Bathing Epidemiology Biology medicine.disease_cause Virus Disease Outbreaks Astrovirus 03 medical and health sciences 0302 clinical medicine Surveys and Questionnaires medicine Humans 030212 general & internal medicine Child Finland Swimming Aged Aged 80 and over 0303 health sciences Reverse Transcriptase Polymerase Chain Reaction 030306 microbiology Norovirus Shock chlorination Infant Outbreak Middle Aged Contamination biology.organism_classification Virology Gastroenteritis 3. Good health Infectious Diseases Child Preschool Gastroenteritis outbreak RNA Viral Female Water Microbiology Mamastrovirus Research Article |
Zdroj: | Epidemiology and Infection. 132:737-743 |
ISSN: | 1469-4409 0950-2688 |
DOI: | 10.1017/s0950268804002249 |
Popis: | In July 2001, an outbreak of gastroenteritis occurred in Helsinki among children and adults after bathing in an outdoor wading pool. The epidemiological survey revealed that at least 242 persons were affected. Microbiological testing of both patient stool samples and of the pool water revealed the presence of two different gastroenteritis viruses: a norovirus (NV) and an astrovirus. Amplicon sequencing of the NV samples showed nucleotide sequence identity between the virus from patients and the water. After changing the pool water and the sand at the bottom of the pool followed by shock chlorination, no virus could be detected in the water. However, NV was continuously detected in the water outlet well as much as 8 months after the incident. Here we show how molecular methods aided in tracing the source of the epidemic and in finding the causative pathogens both in patients and in the environment. |
Databáze: | OpenAIRE |
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