Preparation of β-CD-Ellagic Acid Microspheres and Their Effects on HepG2 Cell Proliferation
| Autor: | Meiling Kang, Chengshi Ding, Dongfeng Ming, Hongkai Wang, Yingxia Zhang, Jing Ma, Zhongjing Tian |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
Cell Survival
Surface Properties Pharmaceutical Science Beta-Cyclodextrins Antineoplastic Agents Apoptosis 02 engineering and technology Cell morphology Article Analytical Chemistry HepG2 cell proliferations lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Ellagic Acid lcsh:Organic chemistry Drug Discovery Humans β-CD-ellagic acid microspheres Physical and Theoretical Chemistry Particle Size Cell Proliferation Gel electrophoresis preparation Drug Carriers Chromatography Dose-Response Relationship Drug Chemistry Cell growth identification Organic Chemistry beta-Cyclodextrins Hep G2 Cells 021001 nanoscience & nanotechnology Microspheres Staining Drug Liberation Kinetics Chemistry (miscellaneous) 030220 oncology & carcinogenesis Molecular Medicine Tumor Suppressor Protein p53 0210 nano-technology Drug carrier Ellagic acid DNA Damage |
| Zdroj: | Molecules; Volume 22; Issue 12; Pages: 2175 Molecules, Vol 22, Iss 12, p 2175 (2017) Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry |
| ISSN: | 1420-3049 |
| DOI: | 10.3390/molecules22122175 |
| Popis: | Objective: In this study, β-cyclodextrin (β-CD) was chosen as the coating for ellagic acid to prepare ellagic acid microspheres, and the effect of microspheres on the growth of HepG2 cells was observed. Methods: Scanning electron microscopy, infrared spectroscopy, and release rate analysis were used to identify the formation of ellagic acid microspheres. Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of different concentrations of ellagic acid microspheres on tumor cell proliferation at 6, 12, 24 and 36 h, and cell morphology and quantity were observed using hematoxylin-eosin (HE) staining. Single-cell gel electrophoresis was used to observe the effect of ellagic acid microspheres on the DNA damage of HepG2 cells, and the Olive tail moment and the mRNA expression of tumor suppressor protein gene p53 was measured. Results: β-CD could be used as wrapping material of ellagic acid to prepare ellagic acid microspheres. HepG2 cell proliferation could be inhibited by 0.1, 0.3 and 0.5 g/L of ellagic acid microspheres in a dose- and time-dependent manner, and the mechanism of proliferation inhibition was related to DNA damage and cell apoptosis. Conclusion: Preparing ellagic acid microspheres with β-CD is feasible, and ellagic acid microspheres have potential therapeutic value (anticancer). |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|