Dendrimer-RNA nanoparticles generate protective immunity against lethal Ebola, H1N1 influenza, and Toxoplasma gondii challenges with a single dose
| Autor: | Sina Bavari, Jasdave S. Chahal, Saima M. Sidik, Christopher L. Cooper, Omar F. Khan, Daniel G. Anderson, Sebastian Lourido, Robert Langer, Hidde L. Ploegh, Lucas D. Tilley, Jonathan K. Tsosie, Justine S. McPartlan |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Multidisciplinary Ebola virus RNA Toxoplasma gondii 02 engineering and technology Biology 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease_cause Virology Microbiology 03 medical and health sciences 030104 developmental biology Antigen PNAS Plus Dendrimer medicine Replicon 0210 nano-technology Pathogen CD8 |
| Popis: | Significance To respond better to evolving pathogens, sudden outbreaks, and individual patient needs, a flexible, safe, and efficient vaccine platform amenable to rapid production near the point of care is required. To this end, we created a fully synthetic, single-dose, adjuvant-free nanoparticle vaccine platform wherein modified dendrimer molecules nanoencapsulate antigen-expressing replicon mRNAs. Vaccines can be multiplexed and formed with multiple antigen-expressing replicons. After a single immunization, the rapid-production, contaminant-free vaccines elicit vital CD8 + T-cell and antibody responses that fully protect against lethal exposures to several deadly pathogens, including Ebola virus, H1N1 influenza, and Toxoplasma gondii . We believe this technology may allow for rapid-response vaccines with broad efficacy that reduce the number and frequency of vaccinations, and healthcare worker burden. |
| Databáze: | OpenAIRE |
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