Changes in cytosolic resting ionized calcium level and in calcium transients during in vitro development of normal and Duchenne muscular dystrophy cultured skeletal muscle measured by laser cytofluorimetry using indo-1
| Autor: | Gérard Duport, Y. Rideau, M. Rivet-Bastide, Nathalie Imbert, Guy Raymond, Christian Cognard |
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| Rok vydání: | 1993 |
| Předmět: |
Adult
medicine.medical_specialty Indoles Adolescent Physiology chemistry.chemical_element Calcium Biology Indo-1 Calcium in biology Muscular Dystrophies chemistry.chemical_compound Cytosol Internal medicine Caffeine medicine Myocyte Humans Fluorometry Child Molecular Biology Cells Cultured Calcium metabolism Myogenesis Lasers Muscles Skeletal muscle Depolarization Cell Biology Acetylcholine medicine.anatomical_structure Endocrinology chemistry Potassium |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0143-4160 |
| Popis: | Intracellular calcium activity was recorded during in vitro myogenesis of human normal and DMD muscle, using the calcium probe Indo-1 under laser illumination, at rest and during different kinds of stimulation (acetylcholine, high K+, caffeine). In myoblasts, the resting intracellular calcium level was significantly larger in DMD cells (89 +/- 9 nM; n = 40 vs 37 +/- 5 nM; n = 22) but there was no significant difference in myotubes, after fusion (44 +/- 4 nM; n = 34 vs 36 +/- 4 nM; n = 52). A similar evolution was observed in cells cultured from FSH biopsies. The amplitude of ACh- and high K(+)-induced calcium transients was significantly halved in DMD myotubes as compared to control ones and non-significantly decreased for caffeine responses. Some alterations in the kinetics of responses were observed in DMD muscle: the rising phases of ACh- and high K(+)-elicited transients and the decaying phase of the ACh-responses were significantly slowed down. It is concluded that: (i) in aneurally cultured human muscle, an increase in the basal level of internal calcium can occur at early stages of myogenesis before the expression of the dystrophin gene; and (ii) the changes in calcium transients induced by depolarization or direct stimulation of sarcoplasmic reticulum are not susceptible of inducing a calcium overload in DMD cells. |
| Databáze: | OpenAIRE |
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