Cell culture (Vero) derived whole virus (H5N1) vaccine based on wild-type virus strain induces cross-protective immune responses
| Autor: | Martin Spruth, Ian Livey, Brian A. Crowe, Leopold Grillberger, Marijan Gerencer, P. Noel Barrett, Wolfgang Mundt, Falko G. Falkner, M. Keith Howard, Christa Tauer, Manfred Reiter, Walter Wodal, Helga Savidis-Dacho, Ines Mayerhofer, Peter Brühl, Otfried Kistner |
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| Rok vydání: | 2006 |
| Předmět: |
H5N1 vaccine
Influenza vaccine Guinea Pigs Biology Cross Reactions medicine.disease_cause Recombinant virus Virus Article Microbiology Mice Orthomyxoviridae Infections Chlorocebus aethiops medicine Influenza A virus Animals Vero Cells Duck embryo vaccine General Veterinary General Immunology and Microbiology Influenza A Virus H5N1 Subtype Public Health Environmental and Occupational Health T-Lymphocytes Helper-Inducer Virology Influenza A virus subtype H5N1 Infectious Diseases Vaccines Inactivated Influenza Vaccines Vero cell Molecular Medicine |
| Zdroj: | Vaccine. 25(32) |
| ISSN: | 0264-410X |
| Popis: | The rapid spread and the transmission to humans of avian influenza virus (H5N1) have induced world-wide fears of a new pandemic and raised concerns over the ability of standard influenza vaccine production methods to rapidly supply sufficient amounts of an effective vaccine. We report here on a robust and flexible strategy which uses wild-type virus grown in a continuous cell culture (Vero) system to produce an inactivated whole virus vaccine. Candidate vaccines based on clade 1 and clade 2 influenza H5N1 strains were developed and demonstrated to be highly immunogenic in animal models. The vaccines induce cross-neutralising antibodies, highly cross-reactive T-cell responses and are protective in a mouse challenge model not only against the homologous virus but also against other H5N1 strains, including those from another clade. These data indicate that cell culture-grown whole virus vaccines, based on the wild-type virus, allow the rapid high yield production of a candidate pandemic vaccine. |
| Databáze: | OpenAIRE |
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