Full Genome Nobecovirus Sequences From Malagasy Fruit Bats Define a Unique Evolutionary History for This Coronavirus Clade

Autor: Gwenddolen Kettenburg, Amy Kistler, Hafaliana Christian Ranaivoson, Vida Ahyong, Angelo Andrianiaina, Santino Andry, Joseph L. DeRisi, Anecia Gentles, Vololoniaina Raharinosy, Tsiry Hasina Randriambolamanantsoa, Ny Anjara Fifi Ravelomanantsoa, Cristina M. Tato, Philippe Dussart, Jean-Michel Heraud, Cara E. Brook
Přispěvatelé: University of Chicago, Chan Zuckerberg BioHub [San Francisco, CA], Université d'Antananarivo, Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP), University of Georgia [USA], Institut Pasteur de Dakar, Research was funded by the National Institutes of Health (1R01AI129822-01 grant to J-MH, PD, and CB), DARPA (PREEMPT Program Cooperative Agreement no. D18AC00031 to CB), the Bill and Melinda Gates Foundation (GCE/ID OPP1211841 to CB and J-MH), the Adolph C. and Mary Sprague Miller Institute for Basic Research in Science (postdoctoral fellowship to CB), the Branco Weiss Society in Science (fellowship to CB), and the Chan Zuckerberg Biohub.
Jazyk: angličtina
Rok vydání: 2022
Předmět:
bat-borne coronavirus
Vaccine Related
[SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases
Chiroptera
Biodefense
Genetics
Madagascar
Animals
Humans
Phylogeny
[SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases
SARS-CoV-2
Prevention
Public Health
Environmental and Occupational Health
COVID-19
[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry
Molecular Biology/Molecular biology
zoonosis
Nobecovirus
recombination
Emerging Infectious Diseases
Infectious Diseases
Severe acute respiratory syndrome-related coronavirus
[SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology
Public Health and Health Services
[SDV.SPEE]Life Sciences [q-bio]/Santé publique et épidémiologie
Public aspects of medicine
RA1-1270
Infection
Biotechnology
Zdroj: Frontiers in Public Health
Frontiers in Public Health, Frontiers Media S.A., 2022, 10, pp.786060. ⟨10.3389/fpubh.2022.786060⟩
Frontiers in Public Health, Vol 10 (2022)
ISSN: 2296-2565
Popis: Bats are natural reservoirs for both Alpha- and Betacoronaviruses and the hypothesized original hosts of five of seven known zoonotic coronaviruses. To date, the vast majority of bat coronavirus research has been concentrated in Asia, though coronaviruses are globally distributed; indeed, SARS-CoV and SARS-CoV-2-related Betacoronaviruses in the subgenus Sarbecovirus have been identified circulating in Rhinolophid bats in both Africa and Europe, despite the relative dearth of surveillance in these regions. As part of a long-term study examining the dynamics of potentially zoonotic viruses in three species of endemic Madagascar fruit bat (Pteropus rufus, Eidolon dupreanum, Rousettus madagascariensis), we carried out metagenomic Next Generation Sequencing (mNGS) on urine, throat, and fecal samples obtained from wild-caught individuals. We report detection of RNA derived from Betacoronavirus subgenus Nobecovirus in fecal samples from all three species and describe full genome sequences of novel Nobecoviruses in P. rufus and R. madagascariensis. Phylogenetic analysis indicates the existence of five distinct Nobecovirus clades, one of which is defined by the highly divergent ancestral sequence reported here from P. rufus bats. Madagascar Nobecoviruses derived from P. rufus and R. madagascariensis demonstrate, respectively, Asian and African phylogeographic origins, mirroring those of their fruit bat hosts. Bootscan recombination analysis indicates significant selection has taken place in the spike, nucleocapsid, and NS7 accessory protein regions of the genome for viruses derived from both bat hosts. Madagascar offers a unique phylogeographic nexus of bats and viruses with both Asian and African phylogeographic origins, providing opportunities for unprecedented mixing of viral groups and, potentially, recombination. As fruit bats are handled and consumed widely across Madagascar for subsistence, understanding the landscape of potentially zoonotic coronavirus circulation is essential for mitigation of future zoonotic threats.
Databáze: OpenAIRE
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