An HPLC method for the assay of UDP-glucose pyrophosphorylase and UDP-glucose-4-epimerase in Solieria chordalis (Rhodophyceae)
| Autor: | F. Goulard, E. Deslandes, Jean-Yves Floc'h, M. Diouris |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Uridine Diphosphate Glucose
UDP-glucose 4-epimerase UTP-Glucose-1-Phosphate Uridylyltransferase Uridine Triphosphate Plant Science Biochemistry High-performance liquid chromatography Analytical Chemistry Uridine Diphosphate Galactose chemistry.chemical_compound UDPglucose 4-Epimerase Spectrophotometry Drug Discovery medicine Chromatography High Pressure Liquid Chromatography biology medicine.diagnostic_test Chemistry UTP—glucose-1-phosphate uridylyltransferase Spectrum Analysis Substrate (chemistry) General Medicine Enzyme assay carbohydrates (lipids) Complementary and alternative medicine Rhodophyta biology.protein Uridine diphosphate glucose Molecular Medicine Food Science |
| Zdroj: | Phytochemical analysis : PCA. 12(6) |
| ISSN: | 0958-0344 |
| Popis: | An efficient method to assay both UDP-glucose pyrophosphorylase and UDP-glucose-4-epimerase in a crude extract of the red seaweed, Solieria chordalis is described. The method is based on the direct quantification by reverse-phase high-performance liquid chromatography of the UDP-sugars generated in the reaction mixture. UDP-glucose, UDP-galactose and UTP were detected by spectrophotometry at 254 nm and their recoveries ranged from 97 to 100%. In the course of the reaction, a correlation was observed between the reduction in the area of the substrate peak and the occurrence of product peak(s). This highly reproducible method for enzyme assay is fast since no intermediate reaction mixture is required. |
| Databáze: | OpenAIRE |
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