Differential Therapeutic Effect of Extracellular Vesicles Derived by Bone Marrow and Adipose Mesenchymal Stem Cells on Wound Healing of Diabetic Ulcers and Correlation to Their Cargoes
| Autor: | Gabriele Togliatto, Marta Tapparo, Tatiana Lopatina, Maria Felice Brizzi, Federica Negro, Cristina Grange, Elena Ceccotti, Massimo Cedrino, Chiara Gai, Giovanni Camussi, Federico Figliolini, Federica Collino, Margherita Alba Carlotta Pomatto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
bone marrow
Angiogenesis Adipose tissue Bone Marrow Cells wound healing mesenchymal Exosomes Exosome Catalysis Article Inorganic Chemistry Diabetes Complications lcsh:Chemistry MSC Extracellular Vesicles Mice microRNA medicine Animals exosome Physical and Theoretical Chemistry cargo Molecular Biology lcsh:QH301-705.5 Spectroscopy Ulcer adipose therapy diabetes Chemistry Gene Expression Profiling Adipose Bone marrow Cargo Diabetes Extracellular vesicle Mesenchymal Therapy Wound healing Organic Chemistry Mesenchymal stem cell Mesenchymal Stem Cells General Medicine Flow Cytometry Immunohistochemistry Computer Science Applications Cell biology medicine.anatomical_structure Adipose Tissue lcsh:Biology (General) lcsh:QD1-999 extracellular vesicle |
| Zdroj: | International Journal of Molecular Sciences International Journal of Molecular Sciences, Vol 22, Iss 3851, p 3851 (2021) Volume 22 Issue 8 |
| ISSN: | 1422-0067 |
| Popis: | Extracellular vesicles (EVs) derived from mesenchymal stem cells isolated from both bone marrow (BMSCs) and adipose tissue (ADSCs) show potential therapeutic effects. These vesicles often show a similar beneficial effect on tissue regeneration, but in some contexts, they exert different biological properties. To date, a comparison of their molecular cargo that could explain the different biological effect is not available. Here, we demonstrated that ADSC-EVs, and not BMSC-EVs, promote wound healing on a murine model of diabetic wounds. Besides a general similarity, the bioinformatic analysis of their protein and miRNA cargo highlighted important differences between these two types of EVs. Molecules present exclusively in ADSC-EVs were highly correlated to angiogenesis, whereas those expressed in BMSC-EVs were preferentially involved in cellular proliferation. Finally, in vitro analysis confirmed that both ADSC and BMSC-EVs exploited beneficial effect on cells involved in skin wound healing such as fibroblasts, keratinocytes and endothelial cells, but through different cellular processes. Consistent with the bioinformatic analyses, BMSC-EVs were shown to mainly promote proliferation, whereas ADSC-EVs demonstrated a major effect on angiogenesis. Taken together, these results provide deeper comparative information on the cargo of ADSC-EVs and BMSC-EVs and the impact on regenerative processes essential for diabetic wound healing. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |