Lymph Node Fine-Needle Cytology: Beyond Flow Cytometry
| Autor: | Pio Zeppa, Anna Lucia Peluso, Antonio Ieni, Chiara Mignogna |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Lymphoma Biopsy Translocation Genetic law.invention 0302 clinical medicine Immunophenotyping law Diagnosis In Situ Hybridization Fluorescence In Situ Hybridization Polymerase chain reaction Sanger sequencing Comparative Genomic Hybridization medicine.diagnostic_test Lymphoma Non-Hodgkin General Medicine Flow Cytometry 030220 oncology & carcinogenesis symbols Histology Cytodiagnosis Biopsy Fine-Needle IGH/T-cell receptor clonality tests Non-Hodgkin Translocation Chromogenic in situ hybridization Fluorescence DNA sequencing Pathology and Forensic Medicine Diagnosis Differential Lymph node cytology 03 medical and health sciences symbols.namesake Genetic medicine Humans Fine-needle cytology Flow cytometry High-Throughput technologies Next-generation sequencing Lymph Nodes business.industry High-throughput technologies 2734 Molecular biology Gene expression profiling 030104 developmental biology Differential Fine-Needle business Fluorescence in situ hybridization Comparative genomic hybridization |
| Zdroj: | Acta Cytologica. 60:372-384 |
| ISSN: | 1938-2650 0001-5547 |
| DOI: | 10.1159/000447734 |
| Popis: | Lymph node (LN) fine-needle cytology (FNC) coupled with flow cytometry immunophenotyping provides relevant information for the diagnosis of non-Hodgkin lymphoma (NHL). Numerous studies have shown FNC samples to be suitable for different molecular procedures; in this review, some of the molecular procedures most commonly employed for NHL are briefly described and evaluated in this perspective. Fluorescence in situ hybridization and chromogenic in situ hybridization are briefly described. Polymerase chain reaction (PCR)-based assays are used to identify and quantify mutations and translocations, namely immunoglobulin (IGH) and T-cell receptor rearrangements by clonality testing and IGVH somatic hypermutations either by Sanger sequencing, single-strand conformational polymorphisms or RT-PCR strategies. High-throughput technologies (HTT) encompass numerous and different diagnostic tools that share the capacity of multiple molecular investigation and sample processing in a fast and reproducible manner. HTT includes gene expression profiling, comparative genomic hybridization, single-nucleotide polymorphism arrays and next-generation sequencing technologies. A brief description of these tools and their potential application to LN FNC is reported. The challenge for FNC will be to achieve new knowledge and apply new technologies to FNC, exploiting its own basic qualities. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|