Expression of Septin 3 Isoforms in Human Brain
Autor: | Masanori Takehashi, Marek Banasik, Hiroe Tsukagoshi-Nagai, Todd Stedeford, Kunihiro Ueda, Toshio Kawamata, Noriaki Kinoshita, Seigo Tanaka |
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Jazyk: | angličtina |
Rok vydání: | 2018 |
Předmět: |
Gene isoform
Cellular differentiation Synaptogenesis Retinoic acid Gene Expression Cell Cycle Proteins Tretinoin macromolecular substances Biology Septin Article Antibodies Cell Line GTP Phosphohydrolases chemistry.chemical_compound Genetics medicine Humans Immunoprecipitation Protein Isoforms RNA Messenger Molecular Biology Temporal cortex Brain Chemistry Immunochemistry Alternative splicing fungi Brain Cell Differentiation Human brain Molecular biology Frontal Lobe Up-Regulation medicine.anatomical_structure chemistry biological phenomena cell phenomena and immunity Septins Protein Binding |
Popis: | Septin 3 is a novel member of the septin subfamily of GTPase domain proteins. Human septin 3 was originally cloned during a screening of genes expressed in human teratocarcinoma cells induced to differentiate with retinoic acid. Alternative splicing of the septin 3 gene transcript produces two isoforms, A and B, in the human brain, though their regional expression and physiological function remain to be determined. The purpose of the present study was to identify the expression patterns of human septin 3 isoforms in normal human brain and a human neuroblastoma cell line, SH-SY5Y, after retinoic acid-induced differentiation. The expression and distribution patterns of septin 3 isoforms A and B were similar and resembled that of another septin, CDCrel-1. Septin 3A and 3B were expressed in normal human brain in a region-specific manner, with the highest level in the temporal cortex and hippocampus and the lowest level in the brainstem regions. Prominent immunoreactivity was observed diffusely in the neocortices in association with neuropils and punctate structures suggestive of synaptic junctions. Immunoprecipitation studies revealed that septin 3A, 3B, and CDCrel-1 form a complex in the frontal cortex of human brain. These findings, taken together, suggest that septin 3A and 3B, along with CDCrel-1, play some fundamental role(s) in synaptogenesis and neuronal development. |
Databáze: | OpenAIRE |
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