Extra-pancreatic expression of the rat islet amyloid polypeptide (amylin) gene
| Autor: | S. Legon, Judith C.W. Mak, S. I. Girgis, J. M. Bhatavdekar, C. G. Nicholl |
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| Rok vydání: | 1992 |
| Předmět: |
Male
Amyloid endocrine system medicine.medical_specialty Gene Expression Amylin Calcitonin gene-related peptide Biology Endocrinology Ganglia Spinal Internal medicine medicine Animals Tissue Distribution RNA Messenger Rats Wistar Pancreas Molecular Biology Antrum Pancreatic hormone Gastrin geography geography.geographical_feature_category digestive oral and skin physiology Pylorus Islet Islet Amyloid Polypeptide Rats medicine.anatomical_structure Gastric Mucosa DNA Probes |
| Zdroj: | Journal of Molecular Endocrinology. 9:157-163 |
| ISSN: | 1479-6813 0952-5041 |
| DOI: | 10.1677/jme.0.0090157 |
| Popis: | Messenger RNA for rat islet amyloid polypeptide (IAPP) has been identified not only in the pancreas but also, in lesser amounts, in preparations from the stomach and dorsal root ganglia. In the stomach, insulin mRNA was not detectable, ruling out possible contamination by pancreatic tissue. Because IAPP and calcitonin gene-related peptide (CGRP) are related and CGRP is present in both stomach and dorsal root ganglia, it was possible that 'IAPP' signals were in fact due to cross-hybridization with CGRP mRNA. A second IAPP probe was constructed which does not cross-react. This probe also detected mRNA in both tissues, confirming the expression of IAPP in both tissues. The regional distribution of IAPP mRNA in the stomach did not parallel that of gastrin mRNA. IAPP mRNA was present in the antrum, centrum and pylorus and, like gastrin, the highest amounts were in the pylorus. However, the ratio between the pylorus and centrum was 3.6:1 for IAPP and 156:1 for gastrin. The effects of dietary manipulation were determined; a period of 48 h of starvation reduced pancreatic IAPP mRNA by approximately 60%, whereas in the stomach there was no significant reduction. If the action of IAPP was hormonal, pancreas and stomach would not be acting in concert. A paracrine role for gastric IAPP therefore seems more likely. |
| Databáze: | OpenAIRE |
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