Ultralow power trapping and fluorescence detection of single particles on an optofluidic chip
| Autor: | Evan J. Lunt, Holger Schmidt, Aaron R. Hawkins, Brian S. Phillips, Sergei Kühn |
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| Rok vydání: | 2010 |
| Předmět: |
Materials science
genetic structures Orders of magnitude (temperature) Optical force Biomedical Engineering Nanoparticle Physics::Optics Bioengineering Nanotechnology Trapping Biochemistry Article Trap (computing) Micromanipulation Biopolymers Electric Power Supplies Condensed Matter::Quantum Gases Optical Devices General Chemistry Equipment Design Micro-Electrical-Mechanical Systems Microfluidic Analytical Techniques Fluorescence Photobleaching eye diseases Equipment Failure Analysis Spectrometry Fluorescence Energy Transfer sense organs Excitation |
| Zdroj: | Lab on a chip. 10(2) |
| ISSN: | 1473-0197 |
| Popis: | The development of on-chip methods to manipulate particles is receiving rapidly increasing attention. All-optical traps offer numerous advantages, but are plagued by large required power levels on the order of hundreds of milliwatts and the inability to act exclusively on individual particles. Here, we demonstrate a fully integrated electro-optical trap for single particles with optical excitation power levels that are five orders of magnitude lower than in conventional optical force traps. The trap is based on spatio-temporal light modulation that is implemented using networks of antiresonant reflecting optical waveguides. We demonstrate the combination of on-chip trapping and fluorescence detection of single microorganisms by studying the photobleaching dynamics of stained DNA in E. coli bacteria. The favorable size scaling facilitates the trapping of single nanoparticles on integrated optofluidic chips. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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