Quantitative Determination of Epa and Dha in Fish Oil Capsules for Cardiovascular Disease Therapy in Indonesia by Gc-Ms
| Autor: | Navy Tiarasari, Ryanto Budiono, Amelia Lorensia, Rivan Virlando Suryadinata |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Organoleptic
030204 cardiovascular system & hematology fish oil Article 03 medical and health sciences Disease therapy 0302 clinical medicine cardiovascular disease medicine 030212 general & internal medicine Food science health care economics and organizations business.industry lcsh:Public aspects of medicine EPA lcsh:RA1-1270 Fish oil medicine.disease Eicosapentaenoic acid Quantitative determination DHA Docosahexaenoic acid lipids (amino acids peptides and proteins) GC-MS Gas chromatography–mass spectrometry business Dyslipidemia |
| Zdroj: | Journal of Public Health Research, Vol 10, Iss 2 (2021) Journal of Public Health Research |
| ISSN: | 2279-9036 |
| DOI: | 10.4081/jphr.2021.2159 |
| Popis: | Background: The consumption of EPA (Eicosapentaenoic acid), and DHA (docosahexaenoic acid), from fish oil, in the long run, has been observed to have a positive impact on patients with coronary heart disease. Fish oil products, with so much EPA and DHA content are available, and have very variable prices. Therefore, as a therapy to be used for long-term treatment, the cost factor is to be considered. Design and methods: This study analyzed the content of EPA and DHA, using GC-MS. The sample to be analyzed was the fish oil that has the lowest price (Product A1), and that of the highest (Product A2). Furthermore, the macroscopic analysis was performed, by observing the physical form including organoleptic and qualitative tests, by reading the fragments identified by EPA and DHA. Results: Clinical trials were conducted on patients (about 46 people), with risk factors and dyslipidemia. Product A1 showed EPA at tR= 15.574 min (relative%= 88.49%, similarity= 95%), and DHA at tR= 21.714 min (relative%= 88.92%, similarity= 93%). Product A2 showed EPA at tR= 28.719 min (relative%= 22.58%, similarity= 89%), and DHA at tR= 32.327 min (relative%= 22.87%, similarity= 90%), which meant that both had EPA and DHA contents, in accordance with their labels. Both products were confirmed to reduce total cholesterol in 4weeks (p=0.000, p= 0.000), with no significant difference in their effectiveness (p=0.652). Conclusion: The results showed that both the A1 and A2 products, had the EPA and DHA contents in accordance with their respective labels. However, with the A2 product having a percentage relatively higher than that of the A1 brand, both are equally very effective. Significance for public health Omega-3 consists of EPA and DPA, that provides significant benefits in lowering total cholesterol, which also, reduces the risk of cardiovascular disease. The omega-3 products in the market have relative percentage variability that affects the purification formulas of EPA and DHA, which often cause very large price variations. Furthermore, it is necessary to identify, whether the price difference also affects its effectiveness. |
| Databáze: | OpenAIRE |
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