Cancer-associated fibroblast-derived CXCL11 modulates hepatocellular carcinoma cell migration and tumor metastasis through the circUBAP2/miR-4756/IFIT1/3 axis
Autor: | Jian Sun, Zhang-Fu Yang, Shuang-Jian Qiu, Yong Yi, Cheng Zhou, Ruo-Yu Guan, Gao Liu, Bao-Ye Sun, Pei-Yun Zhou, Jian Zhou, Jia Fan, Zhu-Tao Wang |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Male
Cancer Research Stromal cell Carcinoma Hepatocellular Lung Neoplasms Immunology Interleukin-1beta Mice Nude medicine.disease_cause Article Metastasis Extracellular matrix Tumour biomarkers Cellular and Molecular Neuroscience Cancer-Associated Fibroblasts Cell Movement Cell Line Tumor Paracrine Communication medicine Gene silencing Animals Humans Neoplasm Invasiveness lcsh:QH573-671 Cancer Adaptor Proteins Signal Transducing Mice Inbred BALB C Chemistry lcsh:Cytology Interleukin-17 Liver Neoplasms Intracellular Signaling Peptides and Proteins RNA-Binding Proteins Cell migration Cell Biology RNA Circular medicine.disease Chemokine CXCL11 Tumor Burden Gene Expression Regulation Neoplastic MicroRNAs Cell culture Cancer cell Cancer research Carcinogenesis Signal Transduction |
Zdroj: | Cell Death and Disease, Vol 12, Iss 3, Pp 1-19 (2021) Cell Death & Disease |
ISSN: | 2041-4889 |
Popis: | Cancer-associated fibroblasts (CAFs) are commonly acquired activated extracellular matrix (ECM)-producing myofibroblasts, a phenotypes with multiple roles in hepatic fibrogenesis and carcinogenesis via crosstalk with cohabitating stromal/cancer cells. Here, we discovered a mechanism whereby CAF-derived cytokines enhance hepatocellular carcinoma (HCC) progression and metastasis by activating the circRNA-miRNA-mRNA axis in tumor cells. CAFs secreted significantly higher levels of CXCL11 than normal fibroblasts (NFs), and CXCL11 also had comparatively higher expressions in HCC tissues, particularly in metastatic tissues, than para-carcinoma tissues. Both CAF-derived and experimentally introduced CXCL11 promoted HCC cell migration. Likewise, CAFs promoted tumor migration in orthotopic models, as shown by an increased number of tumor nodules, whereas CXCL11 silencing triggered a decrease of it. CXCL11 stimulation upregulated circUBAP2 expression, which was significantly higher in HCC tissues than para-carcinoma tissues. Silencing circUBAP2 reversed the effects of CXCL11 on the expression of IL-1β/IL-17 and HCC cell migration. Further downstream, the IFIT1 and IFIT3 levels were significantly upregulated in HCC cells upon CXCL11 stimulation, but downregulated upon circUBAP2 silencing. IFIT1 or IFIT3 silencing reduced the expression of IL-17 and IL-1β, and attenuated the migration capability of HCC cells. Herein, circUBAP2 counteracted miR-4756-mediated inhibition on IFIT1/3 via sponging miR-4756. miR-4756 inhibition reversed the effects induced by circUBAP2 silencing on the IL-17 and IL-1β levels and HCC cell migration. In orthotopic models, miR-4756 inhibition also reversed the effects on metastatic progression induced by silencing circUBAP2. |
Databáze: | OpenAIRE |
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