Clinical Amyloid Typing by Proteomics: Performance Evaluation and Data Sharing between Two Centres
| Autor: | Janet A. Gilbertson, Vittorio Bellotti, Julian D. Gillmore, Francesca Lavatelli, Diana Canetti, Graham W. Taylor, Paola Nocerino, Dario Di Silvestre, Giampaolo Merlini, Andrea Bergamaschi, Pierluigi Mauri, Francesca Brambilla, Nigel B. Rendell |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Proteomics
Amyloid Concordance Pharmaceutical Science amyloid proteomics Amyloidogenic Proteins Computational biology proteomics platforms Article Analytical Chemistry lcsh:QD241-441 03 medical and health sciences Mascot LC-MS/MS raw data exchange lcsh:Organic chemistry Drug Discovery Medicine Humans Typing Physical and Theoretical Chemistry 030304 developmental biology 0303 health sciences business.industry Information Dissemination Amyloidosis 030302 biochemistry & molecular biology Organic Chemistry Computational Biology medicine.disease United Kingdom Data sharing Italy Chemistry (miscellaneous) Proteome proteomics results validation Molecular Medicine business Algorithms Software |
| Zdroj: | Molecules Molecules, Vol 26, Iss 1913, p 1913 (2021) Volume 26 Issue 7 |
| ISSN: | 1420-3049 |
| Popis: | Amyloidosis is a relatively rare human disease caused by the deposition of abnormal protein fibres in the extracellular space of various tissues, impairing their normal function. Proteomic analysis of patients’ biopsies, developed by Dogan and colleagues at the Mayo Clinic, has become crucial for clinical diagnosis and for identifying the amyloid type. Currently, the proteomic approach is routinely used at National Amyloidosis Centre (NAC, London, UK) and Istituto di Tecnologie Biomediche-Consiglio Nazionale delle Ricerche (ITB-CNR, Milan, Italy). Both centres are members of the European Proteomics Amyloid Network (EPAN), which was established with the aim of sharing and discussing best practice in the application of amyloid proteomics. One of the EPAN’s activities was to evaluate the quality and the confidence of the results achieved using different software and algorithms for protein identification. In this paper, we report the comparison of proteomics results obtained by sharing NAC proteomics data with the ITB-CNR centre. Mass spectrometric raw data were analysed using different software platforms including Mascot, Scaffold, Proteome Discoverer, Sequest and bespoke algorithms developed for an accurate and immediate amyloid protein identification. Our study showed a high concordance of the obtained results, suggesting a good accuracy of the different bioinformatics tools used in the respective centres. In conclusion, inter-centre data exchange is a worthwhile approach for testing and validating the performance of software platforms and the accuracy of results, and is particularly important where the proteomics data contribute to a clinical diagnosis. |
| Databáze: | OpenAIRE |
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