Modified Spirulina maxima Pectin Nanoparticles Improve the Developmental Competence of In Vitro Matured Porcine Oocytes
| Autor: | Bereket-Molla Tanga, Seonggyu Bang, Xun Fang, Gyeonghwan Seong, Islam M. Saadeldin, Ghangyong Kim, Shan-Lakmal Edirisinghe, Pantu-Kumar Roy, Do-Hyung Kang, Jongki Cho, Ahmad-Yar Qamar, Mahanama De Zoysa |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
General Veterinary
Veterinary medicine Embryo Glutathione Oocyte medicine.disease_cause porcine In vitro maturation Andrology chemistry.chemical_compound medicine.anatomical_structure chemistry QL1-991 SF600-1100 medicine Somatic cell nuclear transfer Inner cell mass Animal Science and Zoology nanoparticles Blastocyst Spirulina maxima pectin development Zoology Oxidative stress embryos |
| Zdroj: | Animals, Vol 11, Iss 2483, p 2483 (2021) Animals Volume 11 Issue 9 |
| ISSN: | 2076-2615 |
| Popis: | Molecular approaches have been used to determine metabolic substrates involved in the early embryonic processes to provide adequate culture conditions. To investigate the effect of modified Spirulina maxima pectin nanoparticles (MSmPNPs) on oocyte developmental competence, cumulus–oocyte complexes (COCs) retrieved from pig slaughterhouse ovaries were subjected to various concentrations of MSmPNPs (0, 2.5, 5.0, and 10 µg/mL) during in vitro maturation (IVM). In comparison to the control, MSmPNPs-5.0, and MSmPNPs-10 groups, oocytes treated with 2.5 µg/mL MSmPNPs had significantly increased glutathione (GSH) levels and lower levels of reactive oxygen species (ROS). Following parthenogenetic activation, the MSmPNPs-2.5 group had a considerably higher maturation and cleavage rates, blastocyst development, total cell number, and ratio of inner cell mass/trophectoderm (ICM:TE) cells, when compared with those in the control and all other treated groups. Furthermore, similar findings were reported for the developmental competence of somatic cell nuclear transfer (SCNT)-derived embryos. Additionally, the relative quantification of POU5F1, DPPA2, and NDP52 mRNA transcript levels were significantly higher in the MSmPNPs-2.5 group than in the control and other treated groups. Taken together, the current findings suggest that MSmPNP treatment alleviates oxidative stress and enhances the developmental competence of porcine in vitro matured oocytes after parthenogenetic activation and SCNT. |
| Databáze: | OpenAIRE |
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