Weight loss-induced stress in subcutaneous adipose tissue is related to weight regain

Autor: Edwin C. M. Mariman, Klaas R. Westerterp, Nadia J. T. Roumans, Stefan G J A Camps, Johan Renes, Freek G. Bouwman
Přispěvatelé: RS: NUTRIM - R4 - Gene-environment interaction, Promovendi NTM, Humane Biologie, RS: NUTRIM - HB/BW section A, Ondersteunend personeel NTM, RS: NUTRIM - HB/BW section B, RS: NUTRIM - R1 - Metabolic Syndrome
Jazyk: angličtina
Rok vydání: 2016
Předmět:
0301 basic medicine
Gerontology
Male
Weight loss
Calnexin
Biopsy
HSP27 Heat-Shock Proteins
Medicine (miscellaneous)
Adipose tissue
Weight Gain
Body Mass Index
0302 clinical medicine
ENDOPLASMIC-RETICULUM STRESS
Adipocytes
Cells
Cultured
Nutrition and Dietetics
biology
Stress proteins
Genetic Diseases
X-Linked
MEN
Middle Aged
APOPTOSIS
DIFFERENTIATION
Female
medicine.symptom
OBESE SUBJECTS
Adult
Heart Defects
Congenital
EXPRESSION
medicine.medical_specialty
030209 endocrinology & metabolism
Cellular stress
Weight regain
LOSS MAINTENANCE
Gigantism
Mitochondrial Proteins
03 medical and health sciences
Young Adult
Hsp27
In vivo
Stress
Physiological
Heat shock protein
Internal medicine
Intellectual Disability
medicine
Beta-actin
Humans
HEAT-SHOCK PROTEINS
OVERWEIGHT
business.industry
Arrhythmias
Cardiac
Chaperonin 60
medicine.disease
Obesity
Actins
Subcutaneous Fat
Abdominal
Hsp70
BETA-ACTIN
030104 developmental biology
Endocrinology
Glucose
biology.protein
business
Follow-Up Studies
Molecular Chaperones
Zdroj: British Journal of Nutrition, 115(5), 913-920. Cambridge University Press
ISSN: 1475-2662
0007-1145
Popis: Initial successful weight loss is often followed by weight regain after the dietary intervention. Compared with lean people, cellular stress in adipose tissue is increased in obese subjects. However, the relation between cellular stress and the risk for weight regain after weight loss is unclear. Therefore, we determined the expression levels of stress proteins during weight loss and weight maintenance in relation to weight regain. In vivo findings were compared with results from in vitro cultured human Simpson–Golabi–Behmel syndrome (SGBS) adipocytes. In total, eighteen healthy subjects underwent an 8-week diet programme with a 10-month follow-up. Participants were categorised as weight maintainers or weight regainers (WR) depending on their weight changes during the intervention. Abdominal subcutaneous adipose tissue biopsies were obtained before and after the diet and after the follow-up. In vitro differentiated SGBS adipocytes were starved for 96 h with low (0·55 mm) glucose. Levels of stress proteins were determined by Western blotting. WR showed increased expressions of β-actin, calnexin, heat shock protein (HSP) 27, HSP60 and HSP70. Changes of β-actin, HSP27 and HSP70 are linked to HSP60, a proposed key factor in weight regain after weight loss. SGBS adipocytes showed increased levels of β-actin and HSP60 after 96 h of glucose restriction. The increased level of cellular stress proteins in the adipose tissue of WR probably resides in the adipocytes as shown by in vitro experiments. Cellular stress accumulated in adipose tissue during weight loss may be a risk factor for weight regain.
Databáze: OpenAIRE
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