Genetic Diversity in W- and R-type Populations of Pseudocercosporella herpotrichoides Based on DNA Restriction Fragment Length Polymorphisms
| Autor: | N. Cavelier, V. Lind, P. Poupard, U. Frei |
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| Rok vydání: | 1995 |
| Předmět: | |
| Zdroj: | Poupard, P, Cavelier, N, Lind, V & Frei, U K 1995, ' Genetic diversity in W-and R-type populations of Pseudocercosporella herpotrichoides based on DNA restriction fragment length polymorphisms ' J Phytopath 143, p, vol. 143, pp. 99-104 . |
| ISSN: | 1439-0434 0931-1785 |
| DOI: | 10.1111/j.1439-0434.1995.tb00239.x |
| Popis: | Thirty-seven isolates of Pseudocercosporella herpotrichoides belonging to W-type and 31 isolates belonging to R-type were analysed for DNA restriction fragment length polymorphisms (RFLPs). They represent diverse geographic origins and different phenotypes related to sensitivity to fungicides. Total DNA digested with EcoRI was hybridized with 22 random DNA probes from a P. herpotrichoides EcoRI-restricted DNA library. Four probes showing polymorphisms among isolates within W-type and R-type and generating a total of 44 RFLPs were retained for cluster analysis. Two main groups were distinguished corresponding to W- and R-types. The genetic diversity among isolates was greater for the W-type than for the R-type. Using a mitochondrial DNA probe from a known R-type isolate, four and three distinct EcoRI-restricted mitochondrial DNA patterns were identified in W- and R-type isolates, respectively. The variability of profiles within each pathotype confirmed a higher degree of polymorphism in the W-type |
| Databáze: | OpenAIRE |
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