Tilorone hydrochloride—A specific probe for A-T regions of duplex deoxyribonucleic acid
| Autor: | M. Woltersdorf, P. Chandra |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Chemical Phenomena
Base pair Stereochemistry Thymus Gland Tilorone In Vitro Techniques Nucleic Acid Denaturation Biochemistry TILORONE HYDROCHLORIDE chemistry.chemical_compound Polydeoxyribonucleotides medicine Animals Molecule Binding site Pharmacology Fluorenes Chemistry DNA Kinetics Crystallography Duplex (building) Dactinomycin Nucleic acid Cattle Dialysis medicine.drug |
| Zdroj: | Biochemical Pharmacology. 25:877-880 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(76)90306-3 |
| Popis: | The interaction between tilorone hydrochloride and a variety of DNAs and synthetic polydeoxynucleotides was investigated in order to delineate the nucleic acid structural features necessary for binding. The binding parameters (Bapp and Kapp) derived from the Scatchard plots of r (u) vs r (determined by equilibrium dialysis experiments) show a very high specificity of tilorone binding to A-T rich DNAs: no specific binding Scatchard plot (cf. Fig. 1d) of tilorone to poly(dG-dC)· poly(dG-dC) was observed. The Kapp value for poly(dG-dC)'poly(dG-dC) was less by a factor of 10−2, compared with those for A-T containing DNAs. The number of binding sites (Bapp) is correlated linearly to the A-T content of the DNAs. The closest distance between bound tilorone molecules is four base pairs. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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