Quality assurance of immunodiagnostic tests in Australasia
| Autor: | Peter Roberts-Thomson, Robert McEvoy, J Bradley, Susan Jovanovich, R Gale |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Immunofixation
Electrophoresis Weakly positive medicine.medical_specialty Asia Anti-nuclear antibody Quality Assurance Health Care Concordance Immunoglobulins Pilot Projects Immunologic Tests Pathology and Forensic Medicine Internal medicine Medicine Humans Antigens Total protein Radial immunodiffusion biology business.industry Australia Complement C3 Bence Jones protein Proteinuria Antibodies Antinuclear Immunology biology.protein business Laboratories Quality assurance Paraproteins |
| Zdroj: | Flinders University PURE |
| ISSN: | 0031-3025 |
| Popis: | Summary A Royal College of Pathologists of Australasia (RCPA) sponsored Quality Assurance Program (QAP) in Clinical Immunology, involving 128 laboratories over a 1 yr period, revealed the following: • successful participation in the program by 16 overseas laboratories (distant from Australia) • only 30% of laboratories succeeded in returning their results by the scheduled date on every occasion • quantitation of urinary total protein and Bence Jones protein was poor and varied over a log scale • immunofixation was more successful in characterizing urinary paraprotein than immunoelectrophoresis • densitometry of protein electrophoresis appeared the method of choice in quantitating serum paraproteins accurately • nephelometric techniques gave better concordance between laboratories than turbidometric, radial immunodiffusion or agglutination techniques • poor concordance between laboratories in detecting weakly positive antinuclear antibodies (ANA) • some laboratories had difficulties in identifying ANA patterns (only 60% of laboratories correctly identified the anti-centromere pattern) • few laboratories could correctly identify antibodies to extrac-table nuclear antigens (ENA) • flow cytometry gave a smaller dispersion of lymphocyte subpopulation percentages than microscopy A method was established to rank laboratory performance of selected tests over the 1 yr period. Such a comparative ranking scheme may alert laboratories in identifying specific or generalized deficiencies in performance. |
| Databáze: | OpenAIRE |
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