A Transcriptomic Approach to the Metabolism of Tetrapyrrolic Photosensitizers in a Marine Annelid
| Autor: | Ana P. Rodrigo, Pedro M. Costa, A. Jorge Parola, Maria Leonor de Almeida Domingues Santos, Mariaelena D’ambrosio |
|---|---|
| Přispěvatelé: | DCV - Departamento de Ciências da Vida, UCIBIO - Applied Molecular Biosciences Unit, LAQV@REQUIMTE, DQ - Departamento de Química |
| Rok vydání: | 2021 |
| Předmět: |
photodynamic
Annelida Porphyrin metabolism Pharmaceutical Science Organic chemistry Analytical Chemistry chemistry.chemical_compound 0302 clinical medicine QD241-441 bile pigments Drug Discovery Gene Regulatory Networks heme Heme Chromatography High Pressure Liquid chemistry.chemical_classification Biological pigment 0303 health sciences Photosensitizing Agents bioinformatics Bile Pigments Biochemistry Chemistry (miscellaneous) Organ Specificity Molecular Medicine Metabolic Networks and Pathways Bioinformatics Article 03 medical and health sciences Metabolomics Species Specificity Bile pigments Photodynamic Animals SDG 14 - Life Below Water Physical and Theoretical Chemistry Gene 030304 developmental biology porphyrin metabolism Biliverdin Sequence Analysis RNA Gene Expression Profiling Organic Chemistry Polychaeta Metabolic pathway Enzyme chemistry Tetrapyrroles sense organs Epidermis 030217 neurology & neurosurgery |
| Zdroj: | Molecules Molecules, Vol 26, Iss 3924, p 3924 (2021) Volume 26 Issue 13 |
| ISSN: | 1420-3049 |
| Popis: | The past decade has seen growing interest in marine natural pigments for biotechnological applications. One of the most abundant classes of biological pigments is the tetrapyrroles, which are prized targets due their photodynamic properties porphyrins are the best known examples of this group. Many animal porphyrinoids and other tetrapyrroles are produced through heme metabolic pathways, the best known of which are the bile pigments biliverdin and bilirubin. Eulalia is a marine Polychaeta characterized by its bright green coloration resulting from a remarkably wide range of greenish and yellowish tetrapyrroles, some of which have promising photodynamic properties. The present study combined metabolomics based on HPLC-DAD with RNA-seq transcriptomics to investigate the molecular pathways of porphyrinoid metabolism by comparing the worm’s proboscis and epidermis, which display distinct pigmentation patterns. The results showed that pigments are endogenous and seemingly heme-derived. The worm possesses homologs in both organs for genes encoding enzymes involved in heme metabolism such as ALAD, FECH, UROS, and PPOX. However, the findings also indicate that variants of the canonical enzymes of the heme biosynthesis pathway can be species- and organ-specific. These differences between molecular networks contribute to explain not only the differential pigmentation patterns between organs, but also the worm’s variety of novel endogenous tetrapyrrolic compounds. |
| Databáze: | OpenAIRE |
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